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Long-term Monitoring of Oxygen Consumption Rates in Highly Differentiated and Polarized Retinal Pigment Epithelial Cultures

4.7K Views

08:09 min

August 16th, 2024

DOI :

10.3791/67038-v

August 16th, 2024


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Retinal Pigment Epithelium

Chapters in this video

0:00

Introduction

1:49

Setup of Resipher System "Sandwich" to Monitor Oxygen Consumption Rate in Well‐Differentiated RPE Cultures

5:11

Measuring Changes in Mitochondrial Metabolism in Hypoxic RPE Using Resipher System

7:03

Calculating the O2 Concentration at the RPE Monolayer to Determine if Cells are in Hypoxic, Normoxic, or Hyperoxic Conditions

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