Department of Bioengineering and Therapeutic Sciences,
California Institute for Quantitative Biosciences,
UC Berkeley-UCSF Graduate Program in Bioengineering,
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences
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PCR-activated cell sorting for cultivation-free enrichment and sequencing of rare microbes.
PloS one , 2015 | Pubmed ID: 25629401
Microfluidic droplet enrichment for targeted sequencing.
Nucleic acids research Jul, 2015 | Pubmed ID: 25873629
Non-coalescence of oppositely charged droplets in pH-sensitive emulsions.
Proceedings of the National Academy of Sciences of the United States of America Jan, 2012 | Pubmed ID: 22203968
Electrode-free picoinjection of microfluidic drops.
Lab on a chip Oct, 2012 | Pubmed ID: 22930333
Flow focusing geometry generates droplets through a plug and squeeze mechanism.
Lab on a chip Dec, 2012 | Pubmed ID: 23117576
Adhesive-based bonding technique for PDMS microfluidic devices.
Lab on a chip Feb, 2013 | Pubmed ID: 23282717
Picoinjection enables digital detection of RNA with droplet rt-PCR.
PloS one , 2013 | Pubmed ID: 23658657
Ultrahigh-throughput Mammalian single-cell reverse-transcriptase polymerase chain reaction in microfluidic drops.
Analytical chemistry Aug, 2013 | Pubmed ID: 23885761
Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.
Lab on a chip Dec, 2013 | Pubmed ID: 24146020
DNA sequence analysis with droplet-based microfluidics.
Lab on a chip Dec, 2013 | Pubmed ID: 24185402
Block-and-break generation of microdroplets with fixed volume.
Biomicrofluidics , 2013 | Pubmed ID: 24404013
Adding reagent to droplets with controlled rupture of encapsulated double emulsions.
Biomicrofluidics , 2013 | Pubmed ID: 24404045
Generating electric fields in PDMS microfluidic devices with salt water electrodes.
Lab on a chip Mar, 2014 | Pubmed ID: 24671446
Coaxial flow focusing in poly(dimethylsiloxane) microfluidic devices.
Biomicrofluidics Jan, 2014 | Pubmed ID: 24753732
Identification and genetic analysis of cancer cells with PCR-activated cell sorting.
Nucleic acids research , 2014 | Pubmed ID: 25030902
Accurate microfluidic sorting of droplets at 30 kHz.
Lab on a chip Jan, 2015 | Pubmed ID: 25352174
Rapid modulation of droplet composition with pincer microvalves.
Lab on a chip Jan, 2015 | Pubmed ID: 25385230
Compact and modular multicolour fluorescence detector for droplet microfluidics.
Lab on a chip Jul, 2015 | Pubmed ID: 26032595
Dissecting enzyme function with microfluidic-based deep mutational scanning.
Proceedings of the National Academy of Sciences of the United States of America Jun, 2015 | Pubmed ID: 26040002
Patterning microfluidic device wettability with spatially-controlled plasma oxidation.
Lab on a chip Aug, 2015 | Pubmed ID: 26105774
Enhanced sequencing coverage with digital droplet multiple displacement amplification.
Nucleic acids research Apr, 2016 | Pubmed ID: 26704978
Droplet barcoding for massively parallel single-molecule deep sequencing.
Nature communications 06, 2016 | Pubmed ID: 27353563
Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry.
Virology journal 12, 2016 | Pubmed ID: 27906039
PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts.
Journal of virological methods 04, 2017 | Pubmed ID: 28042018
Sequence specific sorting of DNA molecules with FACS using 3dPCR.
Scientific reports 01, 2017 | Pubmed ID: 28051104
Genetic interaction mapping with microfluidic-based single cell sequencing.
PloS one , 2017 | Pubmed ID: 28170417
Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding.
Scientific reports 03, 2017 | Pubmed ID: 28290550
Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.
Lab on a chip 06, 2017 | Pubmed ID: 28540956
Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding.
Nature biotechnology Jul, 2017 | Pubmed ID: 28553940
Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.
Proceedings of the National Academy of Sciences of the United States of America 08, 2017 | Pubmed ID: 28760972
Rapid, chemical-free breaking of microfluidic emulsions with a hand-held antistatic gun.
Biomicrofluidics Jul, 2017 | Pubmed ID: 28794817
RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes.
Nature communications 08, 2017 | Pubmed ID: 28835641
Bulk double emulsification for flow cytometric analysis of microfluidic droplets.
The Analyst Dec, 2017 | Pubmed ID: 29131209
Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis.
Analytical chemistry 01, 2018 | Pubmed ID: 29256243
Concentric electrodes improve microfluidic droplet sorting.
Lab on a chip 02, 2018 | Pubmed ID: 29383336
Combined aptamer and transcriptome sequencing of single cells.
Scientific reports Feb, 2018 | Pubmed ID: 29440771
A comparative analysis of single cell and droplet-based FACS for improving production phenotypes: Riboflavin overproduction in Yarrowia lipolytica.
Metabolic engineering 05, 2018 | Pubmed ID: 29698778
An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.
Scientific reports 05, 2018 | Pubmed ID: 29784937
Particle-Templated Emulsification for Microfluidics-Free Digital Biology.
Analytical chemistry 08, 2018 | Pubmed ID: 30033717
Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA.
Microsystems & nanoengineering , 2017 | Pubmed ID: 30147985
Microfluidic bead encapsulation above 20 kHz with triggered drop formation.
Lab on a chip 12, 2018 | Pubmed ID: 30362490
Operation of Droplet-Microfluidic Devices with a Lab Centrifuge.
Micromachines Sep, 2016 | Pubmed ID: 30404331
Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assay.
Methods in cell biology , 2018 | Pubmed ID: 30473066
Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.
Nature immunology 02, 2019 | Pubmed ID: 30643263
High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq.
Lab on a chip 05, 2019 | Pubmed ID: 31020292
Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.
Scientific reports 07, 2019 | Pubmed ID: 31289365
Uniform, Large-Area, Highly Ordered Peptoid Monolayer and Bilayer Films for Sensing Applications.
Langmuir : the ACS journal of surfaces and colloids 10, 2019 | Pubmed ID: 31603340
Discovery of Stable and Selective Antibody Mimetics from Combinatorial Libraries of Polyvalent, Loop-Functionalized Peptoid Nanosheets.
ACS nano 01, 2020 | Pubmed ID: 31789500
Multiplatform Molecular Profiling Reveals Epigenomic Intratumor Heterogeneity in Ependymoma.
Cell reports 02, 2020 | Pubmed ID: 32023450
Linked optical and gene expression profiling of single cells at high-throughput.
Genome biology 02, 2020 | Pubmed ID: 32093753
Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery.
Nucleic acids research 05, 2020 | Pubmed ID: 32095820
Microfluidic particle zipper enables controlled loading of droplets with distinct particle types.
Lab on a chip 07, 2020 | Pubmed ID: 32531004
Multiplatform genomic profiling and magnetic resonance imaging identify mechanisms underlying intratumor heterogeneity in meningioma.
Nature communications 09, 2020 | Pubmed ID: 32968068
Targeted Single-Cell RNA and DNA Sequencing With Fluorescence-Activated Droplet Merger.
Analytical chemistry 11, 2020 | Pubmed ID: 33049138
Single-cell mutation analysis of clonal evolution in myeloid malignancies.
Nature 11, 2020 | Pubmed ID: 33116311
High-Definition Single-Cell Printing: Cell-by-Cell Fabrication of Biological Structures.
Advanced materials (Deerfield Beach, Fla.) Dec, 2020 | Pubmed ID: 33206435
Networks of High Aspect Ratio Particles to Direct Colloidal Assembly Dynamics and Cellular Interactions.
Advanced functional materials Nov, 2020 | Pubmed ID: 33250685
Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification.
Scientific reports 01, 2021 | Pubmed ID: 33436939
Accurate bulk quantitation of droplet digital PCR.
bioRxiv : the preprint server for biology Jan, 2021 | Pubmed ID: 33469578
High diversity droplet microfluidic libraries generated with a commercial liquid spotter.
Scientific reports Feb, 2021 | Pubmed ID: 33623093
Precision ejection of microfluidic droplets into air with a superhydrophobic outlet.
Lab on a chip Mar, 2021 | Pubmed ID: 33656500
1Department of Pharmaceutical Chemistry, University of California, San Francisco,
2Joint UCSF/UCB Bioengineering Graduate Group, University of California, San Francisco,
3Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco,
4California Institute for Quantitative Biosciences, University of California, San Francisco
Katelyn A. Cabral1,
David M. Patterson2,
Olivia J. Scheideler1,
Russell Cole3,
Adam R. Abate4,5,6,
David V. Schaffer7,8,
Lydia L. Sohn9,
Zev J. Gartner2,6,10
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley,
2Department of Pharmaceutical Chemistry, University of California San Francisco,
3, Scribe Biosciences,
4Department of Bioengineering and Therapeutic Sciences, University of California San Francisco,
5California Institute for Quantitative Biosciences, University of California San Francisco,
6Chan Zuckerberg Biohub, University of California San Francisco,
7Department of Chemical & Biomolecular Engineering, University of California Berkeley,
8Helen Wills Neuroscience Institute, University of California Berkeley,
9Department of Mechanical Engineering, University of California Berkeley,
10Center for Cellular Construction, University of California San Francisco
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco,
2University of California Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco,
3Department of Pharmaceutical Chemistry, University of California San Francisco,
4California Institute for Quantitative Biosciences, University of California San Francisco,
5, Chan Zuckerberg Biohub