Lung Tissue Dissociation and Single Cell Isolation
5:21
Flow Cytometric Lung Immune Cell Niche Analysis
6:27
Results: Representative Immune Cell Population and Chemokine Expression Characterization
8:35
Conclusion
Trascrizione
This protocol can be used to investigate alterations in the pulmonary environment following ischemic stroke. This method allows for the identification of 13 lung immune cell types and 13 different cytokines and chemokines in the same mouse via flo
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This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.