Results: Oviduct Cell Dissociation and Quality Assessment of Segmental RNA
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Conclusion
Trascrizione
Different oviduct segments have distinct physiological functions and are differentially susceptible to pathological change. Identification of the different segments in a very small mouse oviduct is challenging, as is the production of a good yield
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A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.