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Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

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08:50 min

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June 9th, 2022

DOI :

10.3791/64069-v

June 9th, 2022

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1,990 Views

Aisha Adil^1,2, Golnaz Karoubi^1,3, Siba Haykal^1,2,4

¹Latner Thoracic Research Surgical Laboratories, University Health Network, Toronto General Hospital, ²Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, ³Institute of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, ⁴Division of Plastic & Reconstructive Surgery, Department of Surgery, University of Toronto

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Keywords Procurement

Decellularization

Perfusion based Bioreactor

Vascularized Composite Allotransplantation

Immunosuppression

Tissue Immunogenicity

Femoral Vessels

Heparinized Saline

Peristaltic Tubing

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