Applying Mechanical Stimulation to Fruit Fly Embryos Using the Microfluidic Chip
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Results: Experimental Measurement of Embryo Compression Level
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Conclusion
Trascrizione
This protocol explains how to fabricate and use microfluidics technologies to leverage the experimental benefits in sample throughput, automated handling, and the ability to precisely apply mechanical stress while simultaneously visualizing sample
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The present protocol describes the design, fabrication, and characterization of a microfluidic system capable of aligning, immobilizing, and precisely compressing hundreds of Drosophila melanogaster embryos with minimal user intervention. This system enables high-resolution imaging and recovery of samples for post-stimulation analysis and can be scaled to accommodate other multicellular biological systems.