The Micronucleus Assay on Cryopreserved Whole Blood

Our research mainly focuses on examining the impact of ionizing radiation on healthy tissues. We are studying the chromosomal radio sensitivity of individuals with cancer and those with DNA repair disorders. Additionally, we are involved in biological dissymmetry, where we assess the DNA damage induced by exposure to ionizing radiation.

To advance research in our field, we use automated microscopic systems for high throughput analysis. Fresh whole blood samples are the most preferred sample type for the micronucleus assay, as it is easy to obtain with minimal discomfort for the patient. However, the need for immediate processing can be logistically challenging during time constraints.

Freezing of whole blood samples allows repeated analysis without the further need for extra sampling. This procedure is an easy and time-efficient way to store blood samples in large scale and multicenter studies. The protocol of the cryopreservation of whole blood will not only offer advantages for the micronucleus assay, but also for other cytogenetic assays or functional assays that require the proliferation of lymphocytes.