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JoVE Journal

Neuroscience

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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

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13:50 min

April 3rd, 2013

April 3rd, 2013

16,969 Views

0:05

Title

2:03

Fixation

5:31

Immunohistochemistry

11:30

Results: Distribution of Endogenous Ng Molecules in Dendritic Spines

13:03

Conclusion

The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in

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