ニトロセルロース結合アッセイを用いて細菌ヒスチジンキナーゼ自己リン酸化の定量化

0:53

Setting up the 96-well Dot Blot Apparatus

2:02

Reaction Initiation and Quenching

2:57

Spotting of Quenched Reactions on Nitrocellulose

4:51

Nitrocellulose Processing

5:48

Preparing Nitrocellulose Membrane for Scintillation Counting

7:31

Results: Quantification of the Autophosphorylation of V. parahaemolyticus H-NOX Associated Histidine Kinase (Vp1876)

8:35

Conclusion