Establishment of an Extracellular Acidic pH Culture System

0:57

Preparation of Acidic Culture Medium

2:50

Harvesting and Treating Cells

4:31

RNA Isolation

6:30

cDNA Synthesis and Analysis

8:01

Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis

8:49

Conclusion