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JoVE Journal

Immunology and Infection

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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

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09:06 min

February 22nd, 2020

February 22nd, 2020

8,228 Views

0:04

Introduction

0:49

SCV Growth Conditions and Physiological Activation of the Salvage Pathway

1:47

Uronic Acid Carbazole Assay

4:36

ELISA for Alginate Quantitation

6:34

Results: Uronic Acid Carbazole Assay, Anti-Alginate mAb-Based ELISA, and Direct Patient Sample Testing

8:15

Conclusion

文字起こし

The small colony variant or SCV technique allows for selection and growth of small colony variants of Pseudomonas aeruginosa. The method is very definitive and allows for easier selection than normal methods. This protocol also details a safer, mo

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Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.

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