JoVE Journal

Genetics

This content is Open Access.

Embryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus

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05:59 min

May 24th, 2020

May 24th, 2020

5,040 Views

0:04

Introduction

0:51

Alignment of C. quinquefasciatus Pre-blastoderm Stage Embryos

1:46

Embryo Microinjection

3:32

Embryo Recovery and Hatching

4:08

Results: Survival and Mutation Rates of Embryos Injected with Single and Multiplexed gRNAs Targeting White

5:25

Conclusion

文字起こし

This protocol describes microinjection procedures for Culex quinquefasciatus embryos that are optimized to work with CRISPR/Cas9 gene editing tools. This technique can efficiently generate site-specific, heritable, germline mutations that can be used for building genetic technologies in this understudied disease vector.

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