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The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

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04:50 min

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February 8th, 2022

DOI :

10.3791/63088-v

February 8th, 2022

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4,166 Views

Ziyi Wang¹^,², Qinghua Liu³^,⁴, Liang Wang³^,⁵, Robert G. Gilbert¹^,²^,⁶, Mitchell A. Sullivan⁷

¹School of Chemistry and Molecular Biosciences, The University of Queensland, ²Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, ³Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, ⁴Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, ⁵Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, ⁶Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, ⁷Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

章

文字起こし

要約

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Glycogen Storage Disease

Tissue Homogenization

Glycogen Isolation Buffer

Ultracentrifugation

Sucrose Gradient

Ethanol Precipitation

Glycogen Content Determination

Freeze Drying

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