저자는 그녀의 histological 일을 크리스티안 Pahrmann 감사합니다. 기금 소냐 Schrepfer는 독일 Forschungsgemeinschaft (DFG) (SCHR992 / 3 1 SCHR992/4-1)에서 연구 부여를 받았습니다.]

약 250-300그램 무게 쥐 찰스 리버 (Sandhofer 웨그 7 D - 97633 Sulzfeld)에서 구입할 수 있습니다. 이식은 기증자 PVG로 쥐, 그리고 수신자가 같은 ACI의 쥐를 사용하여 수행됩니다. 쥐는 일반 조건, 공급 표준 쥐 차우와 물 광고 libidum 아래에 위치해 있습니다. 모든 수술기구는 사용하기 전에 소독을하고 있습니다. 기증자 준비 : 유도 챔버를 사용하여 isoflurane와 쥐 (2,5-3 %)를 마취. <ol><li> 복부와 흉부 머리를 면도하고 그 뒷면에있는 쥐 장소 및 마취를 유지하기 위해 코와 입을 통해 안면을 넣으십시오. </li><li> 널리 프로보 - 요오드, 다음 사용하는 80 % 에탄올을이 단계를 세 번 반복을 사용하여 복부 및 흉부 영역을 소독. </li><li> 뒷다리가 쥐를 충분 anesthesized되어 있는지 확인하는 곤란 반사를 확인합니다. </li><li> 복부를 열고 복부 대동맥에 구멍을 절단하여 쥐 ...에게서 피를 뽑다. </li><li> 조심스럽게 하강 흉부 대동맥을 찾으려면 흉부를 엽니다. </li><li> 지방, 신경 및 식도와 같은 주변 조직에서 흉부 대동맥을 절개하다. </li><li> 대동맥의 조직 손상없이 couter를 사용하여 대동맥의 1.5cm 조각을 철회. </li><li> 감기 무균 식염수에 철저하게 대동맥 이식을 Perfuse 4에서 그래프트를 저장 ° C. </li></ol> 받는 사람 : 유도 챔버를 사용하여 isoflurane와 쥐 (2,5-3 %)를 마취. 체온은 수술하는 동안 유지됩니다. <ol><li> 복부 영역을 면도하고 마취 동안 건조에서 눈을 방지하기 위해 눈 연고를 적용합니다. </li><li> 의 뒷면에있는 쥐를 삽입하고 마취를 유지하기 위해 코와 입을 통해 안면을 넣으십시오. </li><li> 프로보 - 요오드, 다음 사용하는 80 % 에탄올을이 단계를 세 번 반복을 사용하여 복부 지역을 소독. </li><li> 뒷다리가 쥐를 충분 anesthesized되어 있는지 확인하는 곤란 반사를 확인합니다. </li><li> 복부를 열려면 두 단계의 피부와 근육을 분리 정중선 복부 절개를 수행합니다. </li><li> 예열 생리 moisturized 분말 무료로 글러브에 장내를 놓습니다. 수분의 손실을 방지하기 위해 내장 주변의 장갑을 접으십시오. </li><li> 복부 대동맥을 덮고있는 지방 조직을 제거합니다. </li><li> 대동맥은 혈관의 가지에 대한 손상을하지 않도록주의, 분기점에 infrarenal 지역을 형성 해부하다. </li><li> 경우 대동맥의 필요 ligate 지점. </li><li> 먼저 혈액 흐름을 막을 수있는 해부하는 대동맥의 infrarenal 부분에 microsurgical 클램프를 사용합니다. </li><li> 다음 대동맥의 분기점에 가까운 두 번째 클램프를 놓으십시오. </li><li> 혈액의 흐름이 중지 이제, 대동맥의 짧은 세그먼트를 제거합니다. </li><li> 적절한 길이로 단축하고 간격에 위치, 얼음 0,9 % 멸균 식염수에 보관되고있는 기증자의 대동맥 이식을 가져가라. </li><li> 8-0 prolene 봉합사를 (Ethicon, Norderstedt, 독일)를 사용하여 실행 봉합을 수행하는받는 사람 대동맥에 기증자의 대동맥을 연결합니다. 두개골 엔드 - 투 - 엔드 문합와 함께 시작합니다. </li><li> 말초 엔드 - 투 - 엔드 문합가 완료되면 조심스럽게 말초 클램프를 엽니다. </li><li> 아무 출혈은 말초 클램프를 열 후가없는 경우, 신중하게 두개골 겸자를 엽니다. 봉합에서 출혈의 경우에는, 다시 클램프를 닫습니다 출혈을 찾아 하나의 바늘을 사용하여 출혈을 중지합니다. </li><li> 대동맥의 말초 끝에 보이는 펄스가 있어야합니다. </li><li> 다음 복부에 다시​​ 장내를 이동합니다. </li><li> prewarmed 무균 식염수에 복부를 플러쉬. </li><li> 6-0 prolene 실행 봉합 (Ethicon, Norderstedt, 독일)를 사용하여 복벽의 근육 레이어를 닫습니다. </li><li> 피부를 닫습니다 봉합을 실행 5-0 prolene을 (Ethicon, Norderstedt, 독일)를 사용합니다. </li><li> 쥐가 마취 여전히 있지만, subcutaneously Carprofen을 4-5mg/kg 주입. </li><li> 절차의이 유형에 대한 충분한 진통제를 제공하기 위해 metamizol 3 일 게시물 이식을위한 진통제에 대한 식수 (100ml 당 50mg Metamizol)에 추가됩니다. </li></ol>

<ol>
	<li>Deuse, T., Hoyt, G., Koyanagi, T., Robbins, R. C., Schrepfer, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevention+and+Inhibition+But+Not+Reversion+of+Chronic+Allograft+Vasculopathy+by+FK778.">Prevention and Inhibition But Not Reversion of Chronic Allograft Vasculopathy by FK778.</a> Transplantation. 85, 870-877 (2008).</li><li>Mennander, A., Tiisala, S., Halttunen, J., Yilmaz, S., Paavonen, T., Hayry, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+rejection+in+rat+allografts.+An+experimental+model+for+transplant+arteriosclerosis.">Chronic rejection in rat allografts. An experimental model for transplant arteriosclerosis.</a> Arterioscler Thromb Vasc Biol. 11, 671-680 (1991).</li><li>Ono, K., Lindsey, E. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+technique+of+heart+transplantation+in+rats.">Improved technique of heart transplantation in rats.</a> J Thorac Cardiovasc Surg. 57, 225-225 (1969).</li><li>Poston, R. S., Billingham, M., Hoyt, E. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapamycin+reverses+chronic+graft+vascular+disease+in+a+novel+cardiac+allograft+model.">Rapamycin reverses chronic graft vascular disease in a novel cardiac allograft model.</a> Circulation. 100, 67-100 (1999).</li><li>Schrepfer, S., Deuse, T., Koch-Nolte, F., Krieger, T., Haddad, M., Schäfer, H., Pelletier, M. P., Robbins, R. C., Reichenspurner, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=FK778+in+Experimental+Xenotransplantation:+A+Detailed+Analysis+of+Drug+Efficacy.">FK778 in Experimental Xenotransplantation: A Detailed Analysis of Drug Efficacy.</a> J Heart Lung Transplant. 26, 70-77 (2007).</li></ol>

관심 없음 충돌 선언하지 않습니다.

여러 가지 이유로 우리는 orthotopic 대동맥 이식 모델에게 heterotopic 심장 이식 모델에 비해 TVP의 발전을 조사하는 더 정확한 방법을 발견 : heterotopic 심장 이식 모델은 외과 의사의 능력을 좀 더 복잡하고 오류가 발생하기 쉬운 도전적일 수있는 반면, 수술 기법에 대해서, 대동맥 이식 모델은 엔드 - 투 - 엔드 - 문합의 단독 달성을 필요로하는, 쉽게 가능한 방법을 구체화 두 주요 혈관에 대한 엔?...

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		<th>Material Name</th>
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		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Surgical microscope (magnification 25x)</td>
<td>&nbsp;</td>
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<td>Isoflurane</td>
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<td>shaver</td>
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<td>Provo-Iodine</td>
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<td>ice cold 0.9% saline</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<tr>
<td>prewarmed 0.9% sterile saleine</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>eye ointment</td>
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<td>glove</td>
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<td>microsurgical scissirs</td>
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<td>forceps</td>
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<td>clamps</td>
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<td>8-0 suture (prolene)</td>
<td>&nbsp;</td>
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<td>6-0 suture (prolene)</td>
<td>&nbsp;</td>
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<td>5-0 suture (prolene)</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<tr>
<td>Carprofen</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Metamizol</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Q-tips</td>
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orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy

만성 거부 연구 모델은 이식 vasculopathy (TVP)의 개발 기간 동안 pathobiological 및 pathophysiological 프로세스를 조사하기 위해 필수적입니다. 심장 혈관 만성 거부 연구에 대한 일반적으로 사용되는 동물 모델은 실험실 설치류에서 수행한 heterotopic 심장 이식 모델입니다. 오노와 린지 (3) 자신의 기술을 출판 이후이 모델은 실험에 널리 사용됩니다. 혈관의 연구 결과를 분석하기 위해 심장은 sectioned되어야 모든 선박 측정해야합니다. 심혈관 questionings에서 만성 거부를 조사하기 위해 또 다른 방법은 대동맥 이식 모델 (1, 2)입니다. orthotopic 대동맥 이식 모델에서 대동맥 쉽게 histologically (2) 평가하실 수 있습니다. 급성 혈관 거절 우리가 임상 설정에서 찾을 것과 유사한 CAV의 개발을 방지하지 않는 주요 혼란함을 주죠 요인과 시클 로스 포린 A (CSA) 치료 (4 아니므로 PVG - 투 - ACI 모델, CAV 연구에 특히 유용합니다 ). CSA의 A7 일 기간은 급성 거부를 방지하고 TVP​​의 개발과 함께 장기적인 생존을 달성하기 위해이 모델이 필요합니다. 이 모델은 또한 xenogeneic 모델 (5)에서 급성 세포 거부와 미디어 괴사를 조사하는 데 사용할 수 있습니다.

Watch this Scientific Journal Video about Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy at JoVE.com

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

이 동영상은 쥐에 이식 vasculopathy (TVP)의 개발을 연구하기위한 간단한 모델로 orthotopic 대동맥 이식 모델을 보여줍니다.

Orthotopic 대동맥 이식 : 만성 Vasculopathy의 개발 연구에 생쥐 모델

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

orthotopic-aortic-transplantation-rat-model-to-study-development

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12.0K 조회수.  University Hospital Hamburg. 이 동영상은 쥐에 이식 vasculopathy (TVP)의 개발을 연구하기위한 간단한 모델로 orthotopic 대동맥 이식 모델을 보여줍니다.

비디오: Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel stenting are crucial for translational approaches1,2.
The commonly used animal models include mice, rats, rabbits, and pigs3-5. However, the translation of these models into clinical settings remains difficult, since those biological processes are already studied in animal vessels but never performed before in human research models6,7. In this video we demonstrate a new humanized model to overcome this translational gap. The shown procedure is reproducible, easy, and fast to perform and is suitable to study the development of intimal hyperplasia and the applicability of diverse stents.
This video shows how to perform the stent technique in human vessels followed by transplantation into immunodeficient rats, and identifies the origin of proliferating cells as human.

Human Internal Mammary Artery IMA Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis

This video shows a model to study the development of intimal hyperplasia after stent deployment using a human vessel (IMA) in an immunodeficient rat model.

인간 내유 동맥 (IMA) 이식과 Stenting : 인 Restenosis 스텐 트의 개발을 공부하는 인간 모델

Preclinical in vivo research models to investigate pathobiological and pathophysiological processes in the development of intimal hyperplasia after vessel ...

연구

의학

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and standardized not only for the study of allo-rejection but also between syngeneic strains for examining mechanisms of ischemia-reperfusion injury after lung transplantation. Although the application of the rat and other large animal model 2 contributed significantly to the elucidation of these studies, the scope of those investigations is limited by the scarcity of knockout and transgenic rats. Due to no effective therapies for obliterative bronchiolitis, the leading cause of death in lung transplant patients, there has been an intensive search for pre-clinical models that replicate obliterative bronchiolitis. The tracheal allograft model is the most widely used and may reproduce some of the histopathologic features of obliterative bronchiolitis 3. However, the lack of an intact vasculature with no connection to the recipient's conducting airways, and incomplete pathologic features of obliterative bronchiolitis limit the utility of this model 4. Unlike transplantation of other solid organs, vascularized mouse lung transplants have only recently been reported by Okazaki and colleagues for the first time in 2007 5. Applying the basic principles of the rat lung transplant, our lab initiated the obliterative bronchiolitis model using minor histoincompatible antigen murine orthotopic single-left lung transplants which allows the further study of obliterative bronchiolitis immunopathogenesis6.

Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.

Orthotopic 폐 이식의 Murine 모델에 Obliterative Bronchiolitis의 개발

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and ...

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. This is largely true, but there are a number of important principles that can reduce the difficulty of these procedures and enhance reproducibility. Orthotopic aortic transplantation is an excellent procedure in which to learn these principles because it involves only two end-to-end anastomoses, but requires good suturing technique and handling of the vessels for consistent success. This procedure begins with the procurement of a length of abdominal aorta from a donor animal, followed by division of the native aorta in the recipient. The procured aorta is then placed between the divided ends of the recipient aorta and sutured into place using end-to-end anastomoses. To accomplish this objective successfully requires a high degree of concentration, good tools, a steady hand, and an appreciation of how easily the vasculature of a mouse can be damaged, resulting in thrombosis. Learning these important principles is what occupies most of the beginner's time when learning microsurgery in small rodents. Throughout this protocol, we refer to these important points. This model can be used to study vascular disease in a variety of different experimental systems1-8. In the context shown here, it is most often used for the study of post-transplant vascular disease, a common long-term complication of solid organ transplantation in which intimal hyperplasia occurs within the allograft. The primary advantage of the model is that it facilitates quantitative morphometric analyses and the transplanted vessel lies contiguous to the endogenous vessel, which can serve as an additional control9. The technique shown here is most often used for mice weighing 18-25 grams. We have accumulated most of our experience using the C57BL/6J, BALB/cJ, and C3H/HeJ strains.

We describe a technique in which a section of the abdominal aorta from a mouse is transplanted orthotopically to just below the renal arteries in an allogeneic or syngeneic recipient. This technique can be useful in studies in which transplantation of large arteries of uniform size is deemed advantageous.

혈관 질병의 연구에 대한 마우스의 Orthotopic 대동맥 이식

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as studies on graft preservation and ischemia-reperfusion injury 1,2, immunological responses 3,4, hemodynamics 5,6, and small-for-size syndrome 7. The rat OLT is among the most difficult animal models in experimental surgery and demands advanced microsurgical skills that take a long time to learn. Consequently, the use of this model has been limited. Since the reliability and reproducibility of results are key components of the experiments in which such complex animal models are used, it is essential for surgeons who are involved in rat OLT to be trained in well-standardized and sophisticated procedures for this model.
While various techniques and modifications of OLT in rats have been reported 8 since the first model was described by Lee et al. 9 in 1973, the elimination of the hepatic arterial reconstruction 10 and the introduction of the cuff anastomosis technique by Kamada et al. 11 were a major advancement in this model, because they simplified the reconstruction procedures to a great degree. In the model by Kamada et al., the hepatic rearterialization was also eliminated. Since rats could survive without hepatic arterial flow after liver transplantation, there was considerable controversy over the value of hepatic arterialization. However, the physiological superiority of the arterialized model has been increasingly acknowledged, especially in terms of preserving the bile duct system 8,12 and the liver integrity 8,13,14.
In this article, we present detailed surgical procedures for a rat model of OLT with hepatic arterial reconstruction using a 50% partial graft after ex vivo liver resection. The reconstruction procedures for each vessel and the bile duct are performed by the following methods: a 7-0 polypropylene continuous suture for the supra- and infrahepatic vena cava; a cuff technique for the portal vein; and a stent technique for the hepatic artery and the bile duct.

Orthotopic liver transplantation in rats is an indispensable experimental model for biomedical research. Here we present our surgical procedures for orthotopic rat liver transplantation with hepatic arterial reconstruction using a 50% partial graft.

간 간선 재건과 부분 Orthotopic 간 이식의 쥐 모델에 대한 외과 절차

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

A Model of Chronic Nutrient Infusion in the Rat

Chronic exposure to excessive levels of nutrients is postulated to affect the function of several organs and tissues and to contribute to the development of the many complications associated with obesity and the metabolic syndrome, including type 2 diabetes. To study the mechanisms by which excessive levels of glucose and fatty acids affect the pancreatic beta-cell and the secretion of insulin, we have established a chronic nutrient infusion model in the rat. The procedure consists of catheterizing the right jugular vein and left carotid artery under general anesthesia; allowing a 7-day recuperation period; connecting the catheters to the pumps using a swivel and counterweight system that enables the animal to move freely in the cage; and infusing glucose and/or Intralipid (a soybean oil emulsion which generates a mixture of approximately 80% unsaturated/20% saturated fatty acids when infused with heparin) for 72 hr. This model offers several advantages, including the possibility to finely modulate the target levels of circulating glucose and fatty acids; the option to co-infuse pharmacological compounds; and the relatively short time frame as opposed to dietary models. It can be used to examine the mechanisms of nutrient-induced dysfunction in a variety of organs and to test the effectiveness of drugs in this context.

A protocol for chronic infusions of glucose and Intralipid in rats is described. This model can be used to study the impact of nutrient excess on organ function and physiological parameters.

쥐의 만성 영양 주입의 모델

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

쥐의 각막 이식 : 고체 장기 이식의 가장 일반적인 형태를 연구하기위한 모델

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Myography 와이어를 사용 하 여 마우스 Mesenteric 동맥의 isometric 수축 측정

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart failure. Multiple small-animal models of heart transplantation have been used to study the acute and long-term effects of novel therapies. However, few are tested and demonstrated success in clinical trials. It is of critical importance to evaluate new therapies in a clinically relevant large-animal model for efficient and reliable translation of basic studies&#39;&#160;findings. Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and previously used to investigate novel cardioprotective strategies. This procedure focuses on acute ischemia-reperfusion injury and is a reliable method to investigate novel interventions which have been tested and validated in smaller experimental models, such as the murine model. We demonstrate its usefulness in assessing cardiac performance during the early post-transplantation period and other potential possibilities enabled by the model.

Here, we describe a pre-clinical large-animal (porcine) model of orthotopic heart transplantation that has been firmly established and utilized to investigate novel cardioprotective strategies.

정형외 심장 이식의 전 임상 돼지 모델

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of renal replacement therapy. Over the past few decades, the rat kidney transplantation model has been used to study the immunological phenomena of rejection and tolerance. This model has become an indispensable tool to test new immunomodulatory pharmaceuticals and regimens prior to proceeding with expensive preclinical large animal studies.
This protocol provides a detailed overview of how to reliably perform orthotopic kidney transplantation in rats. This protocol includes three distinctive steps that increase the probability of success: perfusion of the donor kidney by flushing through the portal vein and the use of a cuff system to anastomose the renal veins and ureters, thereby decreasing cold and warm ischemia times. Using this technique, we have achieved survival rates beyond 6 months with normal serum creatinine in animals with syngeneic or tolerant kidney transplants. Depending on the aim of the study, this model can be modified by pre- or posttransplant treatments to study the acute, chronic, cellular, or antibody-mediated rejection. It is a reproducible, reliable, and cost-effective animal model to study different aspects of kidney transplantation.

The purpose of this manuscript and protocol is to explain and demonstrate in detail the surgical procedure of orthotopic kidney transplantation in rats. This method is simplified to achieve the correct perfusion of the donor kidney and shorten the reperfusion time by using the venous and ureteral cuff anastomosis technique.

직교 쥐 신장 이식: 새롭고 단순화 된 외과 접근

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation of human liver transplantation due to the absence of arterial reconnection. Described here is a modified transplantation procedure that includes the incorporation of hepatic artery (HA) reconnection, leading to a marked improvement in transplant outcomes. With a mean anhepatic time of 12 min and 14 s, HA reconnection results in improved perfusion of the transplanted liver and an increase in long-term recipient survival from 37.5% to 88.2%. This protocol includes the use of 3D-printed cuffs and holders to connect the portal vein and infrahepatic inferior vena cava. It can be implemented for studying multiple aspects of liver transplantation, from immune response and infection to technical aspects of the procedure. By incorporating a simple and practical method for arterial reconnection using a microvascular technique, this modified rat OLT protocol closely mimics aspects of human liver transplantation and will serve as a valuable and clinically relevant research model.

The goal of this study is to modify the rat orthotopic liver transplant model to better represent human liver transplantation and improve recipient survival. The presented method reestablishes hepatic arterial inflow by connecting the donor liver&#39;s common hepatic artery to the recipient liver&#39;s proper hepatic artery.

직교 간 이식의 쥐 모형에 있는 동맥 재연결 후에 감소된 합병증

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...