인물에 대한 샘플 문화를 설정하는 그림을 준비하고 UCLA의 크리스 Reddi에 대한 Iroc 설계에서 Cori 샌더스에 대한 특별 감사합니다. 이 프로젝트에 대한 자금 지원은 HHMI (HHMI 그랜트 번호 52006944)에 의해 제공되었다.

<ol>
	<li>Barker, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> At the Bench: A Laboratory Navigator. , (1998).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Biosafety in Microbiological And Biomedical Laboratories (BMBL). , (2009).</li><li>Bykowski, T., Stevenson, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aseptic+Technique.">Aseptic Technique.</a> Current Protocols in Microbiology. Appendix 4, Appendix 4D (2008).</li><li>Cot&#233;, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aseptic+Technique+for+Cell+Culture.">Aseptic Technique for Cell Culture.</a> Current Protocols in Cell Biology. Chapter 1, Unit 1.3 (2001).</li><li>Grimes, S. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+basic+laboratory+manual+for+the+small-scale+production+and+testing+of+I-2+Newcastle+disease+vaccine.">A basic laboratory manual for the small-scale production and testing of I-2 Newcastle disease vaccine.</a> RAP Publication. AC802/E, 12-13 (2002).</li><li>Guzman, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pipetting:+A+Practical+Guide.">Pipetting: A Practical Guide.</a> The American Biology Teacher. 63 (2), 128-131 (2001).</li><li>Jordan, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> RESEARCH: National Genomics Research Initiative Phage Resource Laboratory Manual. , (2008).</li><li>Sambrook, J., Russell, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Molecular Cloning - A Laboratory Manual. , 978-087969576 (2001).</li><li>Seidman, L. A., Moore, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Basic Laboratory Methods for Biotechnology: Textbook and Laboratory Reference. , (2000).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> PIPETMAN P User's Guide. , (2012).</li></ol>

나는 공개 할게 없다.

무균 기술은 실험실에서 원치 않는 미생물에 의해 오염되는 것을 멸균 솔루션과 문화를 막을 일상적인 절차의 집합을 말합니다. 이러한 기술은 성장 세포를 필요로 실험을위한 필수적입니다. 완전 멸균은 작업 설정이 달성될 수는 없지만, 같은 실험실 표면을 소독 분젠 버너를 사용하여 멸균 필드를 만들고, 공기에 uncapped 문화와 미디어의 노출을 제한, 그러한 병, 튜브 및 유리 pipettes과 같은 물질을 살균 등의 절차, 및 비 멸균 표면 살균 계기의 접촉을 피하는 것이 실험에서 솔루션과 문화를 오염 가능성을 줄여줍니다. 목표는 제 2의 천성이 될하려면 다음 예방 절차를 위해이다; 연구실에 근무하는 동안 이것은 훈련과 연습이 제공됩니다. 이러한 serological pipettes와 마이크 등의 악기를 사용하여 멸균 솔루션과 문화가있는 볼륨 전송ropipettors는 실험실에서 수행 루틴 기법의 여러 유형 중 하나입니다. 다른 실험적인 응용 프로그램은 별개의, 아직 정밀하고 정확한 볼륨을 전송할 수있는기구 부릅니다. micropipettors 솔루션만을 microliter 양의 필요한 분자 생물학 실험에 필수적인 반면 Serological pipettes은 밀리리터 볼륨을 포함한 미디어의 준비를 필요로하는 세포 문화를 준비하는 미생물학 실험실에 사용됩니다. 무균 기술은 이러한 악기와 함께 연습을하면 오염은 액체 또는 실험 형태의 금액에 관계없이 볼륨 전송하는 동안 최소화된다. 이 프로토콜에서 설명하지 않지만, 일반적으로 오염을 방지하기 위해 사용하는 다른 한 방법은 층류 후드 (그림 10) 내에서 작동하는 것입니다. 이 장비는 조직 문화와 BLS-2 이상으로 분류된 미생물과 함께 수행한 실험에 중요합니다. 층류 후드는 HEPA (고효율이 포함되어 있습니다작업 영역을 permeating에서 회의실에서 필터없는 공기를 방지하면서 후드에 흐르는 공기에서 공기로 운반되는 오염 물질을 제거 미립자 공기) 필터. 화염의 열기가 후드의 기능에 필수적인 공기 흐름을 방해하기 때문에 참고, 분젠 버너는 층류 후드 안에서 사용할 수 없습니다. 그것은 실험을 수행할 때 무균 기법의 품질을 확인하는 것이 도움이 될 것입니다. 솔루션을 확인하려면 문화 미디어 실험 조작 중에 오염된받을 수 없어 항상 부정적인 컨트롤을 준비합니다. 예를 들어, 세균성 문화의 성장 국물의 튜브를 준비한다면, 한 튜브만을 무균 미디어를두고 접종하지 않습니다. 같은 실수 튜브에 소개 원치 않는 세포의 성장에서 탁도 등 오염의 징후에 대해 다음 검사 uninoculated 제어 튜브 주사 튜브와 함께 매체를 품어. 제어 튜브가 오염되면 실험 튜브들 가능성뿐만 아니라 오염되며, 실험을 반복해야한다. 이러한 예방책은 모든 실험을 다해야합니다.

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td><td> 댓글 </td></tr><tr><td> LB의 국물 </td><td> Difco </td><td> 244,620 </td><td> 참조 6도 사용할 레시피 </td></tr><tr><td colspan="4"> TE 버퍼 : </td></tr><tr><td> EDTA (에틸렌 다이아 민 테트라 초산) 나트륨 소금의 이수화물 </td><td> 시그마 </td><td> E5134 </td><td></td></tr><tr><td> Trizma-HCL </td><td> 시그마 </td><td> T-3253 </td><td></td></tr><tr><td> CiDecon </td><td> 방사능 오염을 제거 연구소 주식 회사 </td><td> 8504 </td><td> 소독제 </td></tr><tr><td> 에탄올 </td><td> 피셔 과학 </td><td> A406 </td><td> 살균제로 사용 들면, 증류수 70 % (V / V)를 준비 </td></tr></tbody></table>

aseptic laboratory techniques: volume transfers with serological pipettes and micropipettors

미생물은 어디 에나있다 - 공기, 토양, 그리고 인체뿐 아니라 실험실 벤치 및 컴퓨터 키보드와 같은 생명이없는 표면. 미생물의 편재는 실험실의 잠재적인 오염 물질의 풍부한 공급을 만듭니다. 실험의 성공을 보장하기 위해, 장비, 작업 표면에 오염 물질의 수를 최소화해야합니다. 미생물학의 많은 실험 중에서 공통는 세균 세포 또는 바이러스 입자를 포함하는 문화의 측정 및 전송과 관련된 기술입니다. 멸균 작업 공간을 준비 멸균 매체가 필요합니다 (1), (2) 정확하게 설정하고 정확하게 액체의 무균 전송을 위해 악기를 읽고, 그리고 (3) 적절하게, 문화의 flasks, 병 악기를 조작을하지 않는 무균 표면에 연락하거나 훼손없이 이렇게하고하려면 멸균 입력란 내의 튜브. 이 절차를 배우는 것은 훈련과 연습을 요구하고있다. 처음에는 동작이 느리고 신중한 및 무균 t 당분간 목표로 제어되어야벤치에서 작업할 때 제 2의 천성이 될 echnique. 여기 분젠 버너에 의해 만들어 멸균 분야 내에서 serological pipettes 및 micropipettors를 사용하여 볼륨을 측정하는 단계를 소개합니다. 볼륨은 microliters (μl)에서 사용하는 악기에 따라 밀리리터 (ML)로 다양합니다. 일반적으로 양도 액체는 멸균 국물이나 화학 솔루션뿐만 아니라 세균성 문화와 파지 주식을 포함합니다. 이 절차를 수행하여, 학생에게 수 있어야한다 : <ul><li> 분젠 버너 불꽃에서 만든 멸균 분야 내에서 작동합니다. </li><li> 악기 불임을 손상시키지 않고 serological pipettes를 사용하십시오. </li><li> serological pipettes로 액체를 대기음, 정확하게 피펫에 졸업 자국으로 액체에 의해 형성된 초승달 모양을 정렬하여 보정 볼륨을 읽고. </li><li> 문화 병, flasks, 튜브 및 액체 전송 중에 멸균 각각의 모자 보관하십시오. </li><li> 플라스틱 대 유리 serological의 핍에 대해 서로 다른 애플 리케이션을 식별ettes. </li><li> micropipettors에 대한 국가의 정확성 제한. </li><li> 정확하고 정확하게 micropipettors에 볼륨을 설정합니다. </li><li> 제대로 올바른 볼륨을 대기음하고 전송하는 micropipettor에서 첫 번째와 두 번째 정거장을 사용하는 방법을 알아요. </li></ul>

Watch this Scientific Journal Video about Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors at JoVE.com

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

연구실에 근무하면 오염의 소스를 최소화하기 위해 필수적입니다. 무균 기법이 아닌 멸균 표면과 접촉을 피하면서 문화와 시약의 양도를 허용 절차를 말합니다. Serological pipettes 및 micropipettors는 실험에 사용되는 솔루션의 불임을 손상시키지 않고 정확한 볼륨을 측정하는 데 사용됩니다.

무균 실험실 기술 : Serological Pipettes 및 Micropipettors로 볼륨 이동

Microorganisms are everywhere - in the air, soil, and human body as well as on inanimate surfaces like laboratory benches and computer keyboards. The ...

aseptic-laboratory-techniques-volume-transfers-with-serological

Research

JoVE Journal

Biology

161.8K 조회수.  University of California, Los Angeles. 연구실에 근무하면 오염의 소스를 최소화하기 위해 필수적입니다. 무균 기법이 아닌 멸균 표면과 접촉을 피하면서 문화와 시약의 양도를 허용 절차를 말합니다. Serological pipettes 및 micropipettors는 실험에 사용되는 솔루션의 불임을 손상시키지 않고 정확한 볼륨을 측정하는 데 사용됩니다.

비디오: Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Microbial Communities in Nature and Laboratory - Interview

자연과 실험실에서 미생물 커뮤니티 - 인터뷰

연구

생물학

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades. Today, such pipettes are made from glass capillaries using a programmable puller. Such instruments heat the capillary using either a metal filament or a laser and draw out the glass using gravity, a motor or both. Pipettes for patch-clamp recording are formed using only heat and gravity, while sharp electrodes for intracellular recording use a combination of heat, gravity, and a motor. The procedure used to make intracellular recording pipettes is similar to that used to make injection needles for a variety of applications, including cRNA injection into Xenopus oocytes. In general, capillary glass &lt;1.2 mm in diameter is used to make pipettes for patch clamp recording, while narrower glass is used for intracellular recording (outer diameter = 1.0 mm). For each tool, the puller is programmed slightly differently. This video shows how to make both kinds of recording pipettes using pre-established puller programs.

This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

프로그래밍 풀러와 패치 - pipettes와 샤프 전극을 만들기

Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades.  Today, such pipettes are made from glass capillaries ...

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording. We first developed this method for fabricating pipettes suitable for recording from small (&lt;3 m) neuronal cell bodies. The basic principal is similar to glass-blowing and combines air pressure and heat to modify the shape of patch pipettes prepared by a conventional micropipette puller. It can be applied to so-called soft (soda lime) and hard (borosilicate) glasses. Generally speaking, pressure polishing can reduce pipette resistance by 25% without decreasing the diameter of the tip opening (Goodman and Lockery, 2000). It can be applied to virtually any type of glass and requires only the addition of a high-pressure valve and fitting to a microforge. This technique is essential for recording from ultrasmall cells (&lt;5 m) and can also improve single-channel recording by minimizing pipette resistance. The blunt shape is also useful for perforated-patch clamp recording since this tip shape results in a larger membrane bleb available for perforation.

This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.

향상된 패치 - 클램프 녹음 압력 연마 Pipettes

Pressure-polishing is a method for shaping glass pipettes for patch-clamp recording.  We first developed this method for fabricating pipettes suitable for ...

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology used in electrophysiology. The same equipment is used in the membrane model as on live preparations. Some properties of an isolated nerve cord are investigated: nerve action potentials, recruitment of neurons, and responsiveness of the nerve cord to environmental factors.

This is a demonstration of how biological membranes can be understood using electrical models. We also demonstrate procedures for recording action potentials from the ventral nerve cord of the crayfish for student orientated laboratories.

회로 기판과 생물 세포막을 모델링하고 Axons에 전기 신호를 측정 : 학생 실험실 연습을

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology ...

Aseptic Laboratory Techniques: Plating Methods

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern. These procedures can be performed at the laboratory bench, provided they involve non-pathogenic strains of microorganisms (Biosafety Level 1, BSL-1). If working with BSL-2 organisms, then these manipulations must take place in a biosafety cabinet. Consult the most current edition of the Biosafety in Microbiological and Biomedical Laboratories (BMBL) as well as Material Safety Data Sheets (MSDS) for Infectious Substances to determine the biohazard classification as well as the safety precautions and containment facilities required for the microorganism in question. Bacterial strains and phage stocks can be obtained from research investigators, companies, and collections maintained by particular organizations such as the American Type Culture Collection (ATCC). It is recommended that non-pathogenic strains be used when learning the various plating methods. By following the procedures described in this protocol, students should be able to: 
 
&#9679; Perform plating procedures without contaminating media. 
&#9679; Isolate single bacterial colonies by the streak-plating method. 
&#9679; Use pour-plating and spread-plating methods to determine the concentration of bacteria. 
&#9679; Perform soft agar overlays when working with phage. 
&#9679; Transfer bacterial cells from one plate to another using the replica-plating procedure. 
&#9679; Given an experimental task, select the appropriate plating method.

When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.

무균 실험실 기술 : 도금 방법

Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies ...

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

Histology volume reconstruction facilitates the study of 3D shape and volume change of an organ at the level of macrostructures made up of cells. It can also be used to investigate and validate novel techniques and algorithms in volumetric medical imaging and therapies. Creating 3D high-resolution atlases of different organs1,2,3 is another application of histology volume reconstruction. This provides a resource for investigating tissue structures and the spatial relationship between various cellular features. We present an image registration approach for histology volume reconstruction, which uses a set of optical blockface images. The reconstructed histology volume represents a reliable shape of the processed specimen with no propagated post-processing registration error. The Hematoxylin and Eosin (H&#38;E) stained sections of two mouse mammary glands were registered to their corresponding blockface images using boundary points extracted from the edges of the specimen in histology and blockface images. The accuracy of the registration was visually evaluated. The alignment of the macrostructures of the mammary glands was also visually assessed at high resolution.
This study delineates the different steps of this image registration pipeline, ranging from excision of the mammary gland through to 3D histology volume reconstruction. While 2D histology images reveal the structural differences between pairs of sections, 3D histology volume provides the ability to visualize the differences in shape and volume of the mammary glands.

We present an image registration approach for 3-dimensional (3D) histology volume reconstruction, which facilitates the study of the changes of an organ at the level of macrostructures made up of cells . Using this approach, we studied the 3D changes between wild-type and Igfbp7-null mammary glands.

3 차원 조직학 볼륨 및 마우스 유방 땀샘을 연구에의 응용의 재구성

Histology volume reconstruction facilitates the study of 3D shape and volume change of an organ at the level of macrostructures made up of cells. It can ...

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help regulate a diverse range of biological processes. Analysis of EVs using flow cytometry (FCM) has been notoriously difficult due to their small size and lack of discrete populations positive for markers of interest. Methods for EV analysis, while considerably improved over the last decade, are still a work in progress. Unfortunately, there is no one-size-fits-all protocol, and several aspects must be considered when determining the most appropriate method to use. Presented here are several different techniques for processing EVs and two protocols for analyzing EVs using either individual detection or a bead-based approach. The methods described here will assist with eliminating the antibody aggregates commonly found in commercial preparations, increasing signal&#8211;to-noise ratio, and setting gates in a rational fashion that minimizes detection of background fluorescence. The first protocol uses an individual detection method that is especially well suited for analyzing a high volume of clinical samples, while the second protocol uses a bead-based approach to capture and detect smaller EVs and exosomes.

Many different methods exist for the measurement of extracellular vesicles (EVs) using flow cytometry (FCM). Several aspects should be considered when determining the most appropriate method to use. Two protocols for measuring EVs are presented, using either individual detection or a bead-based approach.

유동 세포 계측법을 사용하여 세포 외 소포의 분석을위한 기술

Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help ...

Measuring Pressure Volume Loops in the Mouse

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the mouse provides great potential to explore cardiac function at the molecular level. The mouse&#39;s small size does present some challenges in regards to performing detailed cardiac phenotyping. Miniaturization and other advancements in technology have made many methods of cardiac assessment possible in the mouse. Of these, the simultaneous collection of pressure and volume data provides a detailed picture of cardiac function that is not available through any other modality. Here a detailed procedure for the collection of pressure-volume loop data is described. Included is a discussion of the principles underlying the measurements and the potential sources of error. Anesthetic management and surgical approaches are discussed in great detail as they are both critical to obtaining high quality hemodynamic measurements. The principles of hemodynamic protocol development and relevant aspects of data analysis are also addressed.

This manuscript describes a detailed protocol for the collection of pressure-volume data from the mouse.

마우스에 압력 볼륨 루프 측정

Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the ...

Techniques to Induce and Quantify Cellular Senescence

In response to cellular stress or damage, proliferating cells can induce a specific program that initiates a state of long-term cell-cycle arrest, termed cellular senescence. Accumulation of senescent cells occurs with organismal aging and through continual culturing in vitro. Senescent cells influence many biological processes, including embryonic development, tissue repair and regeneration, tumor suppression, and aging. Hallmarks of senescent cells include, but are not limited to, increased senescence-associated &#946;-galactosidase activity (SA-&#946;-gal); p16INK4A, p53, and p21 levels; higher levels of DNA damage, including &#947;-H2AX; the formation of Senescence-associated Heterochromatin Foci (SAHF); and the acquisition of a Senescence-associated Secretory Phenotype (SASP), a phenomenon characterized by the secretion of a number of pro-inflammatory cytokines and signaling molecules. Here, we describe protocols for both replicative and DNA damage-induced senescence in cultured cells. In addition, we highlight techniques to monitor the senescent phenotype using several senescence-associated markers, including SA-&#946;-gal, &#947;-H2AX and SAHF staining, and to quantify protein and mRNA levels of cell cycle regulators and SASP factors. These methods can be applied to the assessment of senescence in various models and tissues.

Cellular senescence, the irreversible state of cell-cycle arrest, can be induced by various cellular stresses. Here, we describe protocols to induce cellular senescence and methods to assess markers of senescence.

기술은 유도 및 정량화 세포 노화하기

In response to cellular stress or damage, proliferating cells can induce a specific program that initiates a state of long-term cell-cycle arrest, termed ...

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

Segmentation is the process of isolating specific regions or objects within an imaged volume, so that further study can be undertaken on these areas of interest. When considering the analysis of complex biological systems, the segmentation of three-dimensional image data is a time consuming and labor intensive step. With the increased availability of many imaging modalities and with automated data collection schemes, this poses an increased challenge for the modern experimental biologist to move from data to knowledge. This publication describes the use of SuRVoS Workbench, a program designed to address these issues by providing methods to semi-automatically segment complex biological volumetric data. Three datasets of differing magnification and imaging modalities are presented here, each highlighting different strategies of segmenting with SuRVoS. Phase contrast X-ray tomography (microCT) of the fruiting body of a plant is used to demonstrate segmentation using model training, cryo electron tomography (cryoET) of human platelets is used to demonstrate segmentation using super- and megavoxels, and cryo soft X-ray tomography (cryoSXT) of a mammalian cell line is used to demonstrate the label splitting tools. Strategies and parameters for each datatype are also presented. By blending a selection of semi-automatic processes into a single interactive tool, SuRVoS provides several benefits. Overall time to segment volumetric data is reduced by a factor of five when compared to manual segmentation, a mainstay in many image processing fields. This is a significant savings when full manual segmentation can take weeks of effort. Additionally, subjectivity is addressed through the use of computationally identified boundaries, and splitting complex collections of objects by their calculated properties rather than on a case-by-case basis.

Volume Segmentation and Analysis of Biological Materials Using SuRVoS Super-region Volume Segmentation Workbench

Segmentation of three-dimensional data from many imaging techniques is a major bottleneck in analysis of complex biological systems. Here, we describe the use of SuRVoS Workbench to semi-automatically segment volumetric data at various length-scales using example datasets from cryo-electron tomography, cryo soft X-ray tomography, and phase contrast X-ray tomography techniques.

볼륨 세분화 및 SuRVoS (슈퍼 지역 볼륨 세분화) 워크 벤치를 사용 하 여 생물학 물자의 분석

Segmentation is the process of isolating specific regions or objects within an imaged volume, so that further study can be undertaken on these areas of ...