June 29th, 2011
•In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
Tags
Related Videos
Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
Generation of Stable Transgenic C. elegans Using Microinjection
Microinjection of Xenopus Laevis Oocytes
Generation of Transgenic C. elegans by Biolistic Transformation
Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells
Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
Synthesis of an In vivo MRI-detectable Apoptosis Probe
Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
Copyright © 2024 MyJoVE Corporation. 판권 소유