August 8th, 2013
•Total cellular RNA provides a poor template for studying short-term changes in RNA synthesis and decay as well as the kinetics of RNA processing. Here, we describe metabolic labeling of newly transcribed RNA with 4-thiouridine followed by thiol-specific biotinylation and purification of newly transcribed RNA allowing to overcome these limitations.
Tags
Related Videos
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Induction and Testing of Hypoxia in Cell Culture
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
Measurement of Metabolic Rate in Drosophila using Respirometry
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Copyright © 2024 MyJoVE Corporation. 판권 소유