July 30th, 2015
•Studying medullary thymic epithelial cells in vitro has been largely unsuccessful, as current 2D culture systems do not mimic the in vivo scenario. The 3D culture system described herein - a modified skin organotypic culture model - has proven superior in recapitulating mTEC proliferation, differentiation and maintenance of promiscuous gene expression.
Tags
Related Videos
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유