Results: Representative FACS and RNA Extraction of Dissociated Ganglia Cells
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Conclusion
필기록
The overall goal of this experiment is to label and isolate the airway-innervating nodose neurons of the vagus nerve. This method can help answer key questions in the Peripheral Neurobiology field. Such as, what identifying properties do airway-in
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Organ specific sensory neurons are difficult to identify. Fast Blue tracing is used to identify nodose neurons innervating the airways for cell sorting. Sorted nodose neurons are used to extract high quality ribonucleic acid (RNA) for sequencing. Using this protocol, gene expression of airway specific neurons is determined.