October 18th, 2016
•This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
Related Videos
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유