The overall goal of this protocol is to genetically modify the smut fungus, ustilago maydis. This strain generation method allows functional characterization of genes in a variety of ways. For example, we can delete the genes, we can exchange the
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We describe a robust gene replacement strategy to genetically manipulate the smut fungus Ustilago maydis. This protocol explains how to generate deletion mutants to investigate infection phenotypes. It can be extended to modify genes in any desired way, e.g., by adding a sequence encoding a fluorescent protein tag.