X-ray Fluorescence (XRF)

Overview

Source: Laboratory of Dr. Lydia Finney — Argonne National Laboratory

X-ray fluorescence is an induced, emitted radiation that can be used to generate spectroscopic information. X-ray fluorescence microscopy is a non-destructive imaging technique that uses the induced fluorescence emission of metals to identify and quantify their spatial distribution.

Procedure

1. Preparing the Silicon Nitride Windows

  1. Use reverse tweezers to pick up a window (silicon nitride windows will shatter if dropped).
  2. Place window onto a glass slide, flat side up.
  3. Adhere small pieces of Scotch tape to the sides of the window, and use these to adhere the windows to the bottom of the culture dish.
  4. Sterilize the windows in culture dishes with UV radiation. This can be accomplished with the auto-crosslink setting on a UV-crosslinking cabinet, followed by further UV i

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Results

The X-ray fluorescence map of an adherent cell is shown in Figure 1. Each panel shows the distribution of a particular element (e.g., copper, iron, zinc, etc) over the cell. The panel labeled 's_a' shows the absorption of X-rays.

Figure 1
Figure 1. X-ray fluorescence map of an adherent cell.

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Application and Summary

X-ray fluorescence imaging can be a useful tool in many fields including geosciences, forensic science, materials science, biology, and even in studying our cultural heritage. In materials science, it can help find defects in chips and catalysts made with metals. In cultural heritage work, it has been used to identify poisonous metals in the hair of famous dead people (e.g., Beethoven), and to identify the source of paints used in art. In biology, it is used to study the natural metals that perform important biochemistry

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Tags
X ray FluorescenceXRF SpectroscopyElemental AnalysisNon destructive TechniqueRoom Temperature AnalysisBiological SamplesForensic SamplesEnvironmental SamplesWorks Of ArtPowdersCrystalsLiquidsFluorescent RadiationFluorescence TechniqueX ray PhotonsInner Shell ElectronsSecondary Photon EmissionElement Identification

건너뛰기...

0:00

Overview

1:07

Principles of X-ray Fluorescence

2:37

Preparing the Silicon Nitride Windows

3:09

Plating and Fixing Cells on the Windows

4:10

Generating X-ray Fluorescence Images

5:39

Representative Results

6:09

Applications

7:26

Summary

이 컬렉션의 비디오:

article

Now Playing

X-ray Fluorescence (XRF)

Analytical Chemistry

25.3K Views

article

분석 특성화를 위한 샘플 준비

Analytical Chemistry

83.7K Views

article

내부 표준

Analytical Chemistry

203.5K Views

article

표준 추가 방법

Analytical Chemistry

318.9K Views

article

교정 곡선

Analytical Chemistry

789.1K Views

article

자외선 눈에 보이는 (UV-Vis) 분광법

Analytical Chemistry

616.6K Views

article

화학 분석을 위한 라만 분광법

Analytical Chemistry

50.8K Views

article

화염 이온화 감지를 갖춘 가스 크로마토그래피(GC)

Analytical Chemistry

279.9K Views

article

고성능 액체 크로마토그래피 (HPLC)

Analytical Chemistry

381.9K Views

article

이온 교환 크로마토그래피

Analytical Chemistry

263.0K Views

article

모세관 전기 포레시스 (CE)

Analytical Chemistry

93.0K Views

article

질량 분광법 소개

Analytical Chemistry

111.4K Views

article

스캐닝 전자 현미경 검사법 (SEM)

Analytical Chemistry

86.6K Views

article

전위요스타트/갈바노스타트를 사용하여 지원되는 촉매의 전기화학적 측정

Analytical Chemistry

51.2K Views

article

순환 볼탐량 (CV)

Analytical Chemistry

123.4K Views

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2025 MyJoVE Corporation. 판권 소유