January 6th, 2017
•We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
Related Videos
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
Isolation of Murine Embryonic Hemogenic Endothelial Cells
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Fetal Mouse Cardiovascular Imaging Using a High-frequency Ultrasound (30/45MHZ) System
Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans
Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
Rapid Isolation of Single Cells from Mouse and Human Teeth
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
Copyright © 2024 MyJoVE Corporation. 판권 소유