Preparation of Aortic Ring and Mesenchymal Stromal Cells Co-culture assay
4:49
Flow Cytometry and qPCR
7:09
Results: Aortic Ring Assay with MSC
8:48
Conclusion
필기록
The overall goal of this pre-clinical functional assay is to observe and quantitatively evaluate the angiogenic potential of mesenchymal stem cells intended for regenerative therapy. This method can help answer key questions in the field of vascul
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Here, we present a novel application of the aortic ring assay where prelabelled mesenchymal cells are co-cultured with rat aorta-derived endothelial networks. This novel method allows visualization of Mesenchymal Stromal Cells (MSCs) homing and integration with endothelial networks, quantification of network properties, and evaluation of MSC immunophenotypes and gene expression.