August 15th, 2017
•This protocol outlines a simple method for analyzing calcium signals in plants generated by feeding hemipteran insects, such as aphids. Arabidopsis thaliana transformed with the GFP calcium biosensor GCaMP3 allow for the real-time in vivo imaging of calcium dynamics with a high temporal and spatial resolution.
Related Videos
Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
One-channel Cell-attached Patch-clamp Recording
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
Copyright © 2024 MyJoVE Corporation. 판권 소유