June 4th, 2021
•Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.
Related Videos
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Copyright © 2024 MyJoVE Corporation. 판권 소유