Culturing, Growth, and Target-specific Staining of A. tumefaciens Strains
2:21
Imaging of A. tumefaciens Cells
4:54
Epifluorescence and Time-lapse Microscopy
5:41
Results: Cells Growth of Live Labeled Microbial Cells Imaged with Fluorescence Microscopy
6:47
Conclusion
필기록
The overall goal of this microscopy-based approach is to observe a central processes during growth of bacterial cells. This method enables us to address important questions in bacteriology, such as how essential processes are coordinated during ba
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Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.