Collection of Decidual Tissue from Term Placental Membranes
1:57
Washing and Enzymatic Digestion of the Decidual Tissue
3:56
Generation of Single Cell Suspension
5:22
Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
7:11
Conclusion
필기록
The overall goal of this cell isolation protocol is to develop a method for isolating primary decidual cells with high yield end cell viability. This method can help answer key questions in the field of human reproduction, such as the deciduous ro
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.