July 16th, 2019
•An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.
Related Videos
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
VisualEyes: A Modular Software System for Oculomotor Experimentation
Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
VisioTracker, an Innovative Automated Approach to Oculomotor Analysis
Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish
In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice
Long-term Time Lapse Imaging of Mouse Cochlear Explants
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
Copyright © 2024 MyJoVE Corporation. 판권 소유