October 13th, 2019
•This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.
Tags
Related Videos
Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
Establishment and Characterization of UTI and CAUTI in a Mouse Model
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix
Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats
Investigating Intestinal Barrier Breakdown in Living Organoids
Copyright © 2024 MyJoVE Corporation. 판권 소유