JoVE Logo

로그인

JoVE Journal

Bioengineering

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

live

Speed

×

MEDIA_ELEMENT_ERROR: Format error

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

6.9K Views

10:53 min

October 13th, 2019

October 13th, 2019

6,915 Views

0:04

Title

0:47

Fabrication of Polydimethylsiloxane (PDMS) Stencil and Microchannel

2:39

Preparation of Bottom Glass with Polyacrylamide (PA) Gel

4:41

Micropatterning of Cell Islands and Assembly of PA Gel with PDMS Microchannel

6:14

Integrated Microfluidic System

7:32

Image Acquisition

8:27

Results: Mechanical Interactions of MDCK Cell Islands

9:55

Conclusion

필기록

Collective cell migration is often guided by the gradient of signaling molecules. In this video, we demonstrate how to quantify cellular forces during collective migration guided by biochemical gradient. So, we integrate the microfluidic chip with

이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요

Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient.

당사 웹 사이트에서는 사용자의 경험을 향상시키기 위해 쿠키를 사용합니다.

당사 웹 사이트를 계속 사용하거나 '계속'을 클릭하는 것은 당사 쿠키 수락에 동의하는 것을 의미합니다.