Media Preparation and Extraction of the Sandwich Retinal Explants from the Eyecup
3:02
Results: Observation of Cellular Integrity of Retinal Explants Using H&E, IHC and Analysis of Cytokine Release Using Magnetic Assay
4:53
Conclusion
필기록
This technique preserves all retinal layers and cell types in situ, making it more clinically relevant compared to animal and in vitro models. The main advantage of this protocol is that it minimizes retinal integrity disruption during tissue hand
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This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera.