암의 환자 유래 오가노이드 모델에서 약물 반응을 위한 다중 라이브 셀 이미징

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09:13 min

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January 5th, 2024

DOI :

10.3791/66072-v

January 5th, 2024

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Kaitriana E. Colling*¹^,², Emily L. Symons*¹, Lorenzo Buroni*³, Hiruni K. Sumanasiri¹, Jessica Andrew-Udoh¹, Emily Witt¹^,⁴, Haley A. Losh¹, Abigail M. Morrison¹, Kimberly K. Leslie⁵, Christopher J. Dunnill⁶, Johann S. de Bono³, Kristina W. Thiel¹^,⁷

¹Department of Obstetrics and Gynecology, Carver College of Medicine, University of Iowa, ²Cancer Biology Graduate Program, Carver College of Medicine, University of Iowa, ³The Institute of Cancer Research: and the Royal Marsden NHS Foundation Trust, ⁴Department of Radiation Oncology, Carver College of Medicine, University of Iowa, ⁵Division of Molecular Medicine, Departments of Internal Medicine and Obstetrics and Gynecology, University of New Mexico Comprehensive Cancer Center, University of New Mexico Health Sciences Center, ⁶Agilent Technologies, ⁷Holden Comprehensive Cancer Center, University of Iowa

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Introduction

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Plating Intact Patient‐Derived Organoids and Setting Up Imaging Parameters for Multiplexed Live‐Cell Imaging