Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
831 Views
•
09:15 min
•
July 19th, 2024
DOI :
July 19th, 2024
•Chapters in this video
0:00
Introduction
2:08
Generating Single‐Cell Suspension from Lung Tissue for Identifying Rare Antigen‐Specific T‐Cell Populations
5:25
Enriching the Lung Sample for T Cells via Magnetic Bead Enrichment
7:40
Staining the Antigen‐Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting
Related Videos
13:58
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
17.9K Views
15:42
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
21.6K Views
13:10
In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
11.6K Views
09:47
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
12.9K Views
09:32
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
11.2K Views
06:25
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
9.1K Views
08:37
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
12.2K Views
08:10
Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
8.2K Views
11:31
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
7.8K Views
01:39
A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8+ Memory T Cells
87 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved