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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

DOI :

10.3791/1167-v

March 6th, 2009

March 6th, 2009

21,415 Views

1Tetramer Core Laboratory, Benaroya Research Institute, 2Kwok Laboratory, Benaroya Research Institute, 3Nepom Laboratory, Benaroya Research Institute

This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction.

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CD4 T Cells

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