Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

21.6K Views

15:42 min

March 6th, 2009

DOI :

10.3791/1167-v

March 6th, 2009


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CD4 T Cells

Chapters in this video

0:00

Title

0:09

Introduction

1:05

T Cell Staining

3:21

Reagents and Equipment

5:44

Assemble Materials and Equipment at Workspace

6:04

Step 1: PBMC Isolation

8:51

Step 2: CD4+ Cell Separatioin

11:01

Step 3: In Vitro Expansion Culture

13:34

Step 4: Visualizing T Cells by Tetramer Staining

15:33

Conclusion

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