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Method Article
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
Epilepsy affects nearly 3 million people in the United States and up to 50 million people worldwide. Defined as the occurrence of spontaneous unprovoked seizures, epilepsy can be acquired as a result of an insult to the brain or a genetic mutation. Efforts to model seizures in animals have primarily utilized acquired insults (convulsant drugs, stimulation or brain injury) and genetic manipulations (antisense knockdown, homologous recombination or transgenesis) in rodents. Zebrafish are a vertebrate model system1-3 that could provide a valuable alternative to rodent-based epilepsy research. Zebrafish are used extensively in the study of vertebrate genetics or development, exhibit a high degree of genetic similarity to mammals and express homologs for ~85% of known human single-gene epilepsy mutations. Because of their small size (4-6 mm in length), zebrafish larvae can be maintained in fluid volumes as low as 100 μl during early development and arrayed in multi-well plates. Reagents can be added directly to the solution in which embryos develop, simplifying drug administration and enabling rapid in vivo screening of test compounds4. Synthetic oligonucleotides (morpholinos), mutagenesis, zinc finger nuclease and transgenic approaches can be used to rapidly generate gene knockdown or mutation in zebrafish5-7. These properties afford zebrafish studies an unprecedented statistical power analysis advantage over rodents in the study of neurological disorders such as epilepsy. Because the "gold standard" for epilepsy research is to monitor and analyze the abnormal electrical discharges that originate in a central brain structure (i.e., seizures), a method to efficiently record brain activity in larval zebrafish is described here. This method is an adaptation of conventional extracellular recording techniques and allows for stable long-term monitoring of brain activity in intact zebrafish larvae. Sample recordings are shown for acute seizures induced by bath application of convulsant drugs and spontaneous seizures recorded in a genetically modified fish.
1. Egg Production and Collection
2. Microelectrode Pulling and Preparation
3. Immobilization in Agar
3. Extracellular Field Recording
Examples of electrographic seizure-like discharge recorded in the forebrain of an agar-embedded zebrafish larvae are shown in Figure 1. Large-amplitude multi-spike burst discharge in these samples was evoked by bath application of a convulsant drug, 40 mM pilocarpine (in A; 6 dpf) or 1 mM picrotoxin (in B; 8 dpf). In these recordings, immobilized and agar-embedded zebrafish are continuously monitored for up to 90 min. Fish remain viable under these recording conditions for up to 24 hr. Drugs are added to...
The extracellular recording method presented here enables a very sensitive and rapid analysis of brain activity. These recordings are analogous to electroencephalographic (EEG) monitoring commonly used to evaluate the presence of abnormal electrical discharge (i.e., seizure) in rodent models of epilepsy11 and patients12. Extracellular recordings can be combined with pharmacological manipulations, as shown here. These types of recordings can also be used to evaluate potential epileptic pheno...
No conflicts of interest declared.
The author would like to thank Peter Castro and Matthew Dinday for their early efforts to establish zebrafish in the laboratory. This work was funded by the National Institutes of Health EUREKA grant (#R01NS079214-01).
Name | Company | Catalog Number | Comments |
Agarose low melting | Fisher-Scientific | BP1360-100 | Dissolve in embryo media at 1.2% |
Recording media | Fisher-Scientific | BP3581, P330-3, BP410-1, BP214-500, D16-1, C77-500 | 1 mM NaCl, 2.9 mM KCl, 10 mM HEPES, 1.2 mM MgCl2, 10 mM Dextrose, 2.1 mM CaCl2 pH to approximately 7.3 with 1 N NaOH |
Tricaine | Argent Labs | MS-222 | 0.02% |
α-bungarotoxin | Tocris Bioscience | 2133 | 1 mg/ml |
Capillary glass tubing | Warner Instruments | G120TF-3 | Pull to a resistance of 2 -7 MΩ |
Patch clamp amplifier | Warner Instruments | PC-505B | We use a Warner amplifier in current-clamp mode; Gain set at 2 mV/pA and Bessel filter set at 2K. Comparable models can be used according to manufacturer's instructions. |
Filter/amplifier | Cygnus Technology | FLA-01 | We use a Cygnus pre-amplifier; Gain set at 10-20; Cut-off frequency set at 1-2K; Notch filter IN. Comparable models can be used according to manufacturer's instructions. |
Axon A/D board and Axoscope software | Molecular Devices | Axon Digidata 1320A; Axoscope 8.2 | Data is collected in Axoscope using gap-free acquisition mode; sampling at 10 KHz. Comparable models and programs can be used according to manufacturer's instructions. |
Egg water | Instant Ocean | 3 g Instant Ocean sea salt, 2 ml 0.1% methylene blue in 10 ml deionized water |
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