Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Podsumowanie

The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.

Streszczenie

The efficient transduction of specific genes into natural killer (NK) cells has been a major challenge. Successful transductions are critical to defining the role of the gene of interest in the development, differentiation, and function of NK cells. Recent advances related to chimeric antigen receptors (CARs) in cancer immunotherapy accentuate the need for an efficient method to deliver exogenous genes to effector lymphocytes. The efficiencies of lentiviral-mediated gene transductions into primary human or mouse NK cells remain significantly low, which is a major limiting factor. Recent advances using cationic polymers, such as polybrene, show an improved gene transduction efficiency in T cells. However, these products failed to improve the transduction efficiencies of NK cells. This work shows that dextran, a branched glucan polysaccharide, significantly improves the transduction efficiency of human and mouse primary NK cells. This highly reproducible transduction methodology provides a competent tool for transducing human primary NK cells, which can vastly improve clinical gene delivery applications and thus NK cell-based cancer immunotherapy.

Wprowadzenie

Natural killer (NK) cells are the major lymphocytic population of the innate immune system¹. NK cells function as the first-line defenders of the host immune response against tumors and infections^2,3,4. NK cells also play a central role in the development of tolerance through the secretion of potent cytokines and chemokines⁵. Due to their potent ability to target and eliminate tumor cells, multiple clinical trials are being conducted to evaluate donor-derived human NK cells as an adoptive immunotherapy for cancer^6,7. In contrast to T cells, the developmental biology of NK cells has yet to be well-characterized⁸. This lack of knowledge is partially due to the absence of efficient techniques that deliver genes of interest to mouse or human primary NK cells. For these reasons, most NK-cell studies have been conducted in cell lines, rather than in primary cells. Therefore, the need for a reliable and efficient protocol to transduce primary NK cells with genes of interest is crucial.

The overall goal of this study was to formulate a consistent and reliable method by which primary human or murine NK cells could be transduced with lenti- or retroviruses.

Earlier studies that attempted to address this problem have been performed, largely using the transient transformation of primary NK cells. This includes plasmid transfection^9,10, Epstein-Barr Virus (EBV)/retroviral hybrid vector¹¹, vaccinia vectors^12,13, and Ad5/F35 chimeric adenoviral vectors¹⁴. Despite the modest efficiency of these techniques, the transient nature of transduction makes them unsuitable for the long-term utilization of the genetically modified NK cells. A few recent studies have used retroviral vectors to transduce NK cells, requiring multiple cycles of infection to achieve an acceptable level of gene expression^11,15. In contrast to retroviral vectors, lentiviral vectors can use host-cell nuclear import machinery to translocate the viral pre-integration complex into the nucleus. This is a major limiting factor in the replication of the virus in non-dividing cells, which include primary NK cells.

Interactions between different cell-surface receptors and viral particles permit viral uptake into the cell. The initial engagements between the viral envelope proteins and their cognate host receptors could be limited because of the potential negative charges existing between these two. The rationale behind many transduction techniques is that the addition of cationic polymers, such as polybrene (Pb), protamine sulfate (PS), or dextran, could give a positive charge to the cell-surface receptors and thereby augment the binding of viral envelope proteins. This will increase the fusion efficiency and the uptake of the viral particles by the cells¹⁶. Although it has been reported that Pb or PS can improve gene transfer in T cells¹⁷, their application did not have any effect in the transduction efficiency of primary NK cells. Moreover, a comparative analysis between these reagents using primary NK cells has not been performed. In this study, the transduction efficiencies of the three cationic polymers were compared. The results show that, among these three cationic polymers, only dextran significantly enhances efficient viral transduction into both mouse and human primary NK cells.

Protokół

All animal protocols followed the humane and ethical treatment of animals and were approved by the Institutional Animal Care and Use Committee (IACUC) within the Biomedical Research Center (BRC) of the Medical College of Wisconsin (MCW), Milwaukee, WI. The use of human peripheral blood mononuclear cells (PBMCs) was approved by the Institutional Review Board (IRB) of the Blood Research Institute of the Blood Center of Wisconsin, Milwaukee, WI.

1. Mice, cell lines, and vectors

1. Obtain C57BL/6 mice from commercial vendors. Maintain the mouse colonies in pathogen-free conditions and use female and male mice between the ages of 6 and 12 weeks. Obtain de-identified human PBMCs from IRB-approved sources.
2. Anesthetize the animals witha mixture of 20-30% v/v isoflurane in propylene glycol (1, 2-propanediol, USP grade) to anesthetize the mice. Apply vet ointment to the eyes to prevent dryness while the mice are under anesthesia.
3. To euthanize the animals, perform cervical dislocation after the induction of anesthesia.
   1. Restrain the mice by firmly grasping the base of the tail with one hand. Place a sturdy stick-type pen or the thumb and first finger of the other hand against the back of the neck, at the base of the skull.
   2. To produce the dislocation, push the hand restraining the head of the animal forward and push down while pulling backward with the hand holding the tail base. Verify the effectiveness of dislocation by feeling for a separation of cervical tissue.
4. Keep the animals in the chamber/cage for at least 5 min and remove them only when respiratory activity is absent or when there is a lack of a detectable heartbeat.
5. Obtain K562 and YAC-1 cells from commercial vendors and maintain them in RPMI1640 medium containing 10% heat-inactivated FBS. Test these cell lines periodically to exclude the possibility of mycoplasma contamination.

2. Preparation and titration of lentiviral vectors

1. Culture 5 × 10⁶ 293T cells overnight in a T75 cm² flask containing a solution of 20 mL of RPMI1640 medium with 10% FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, 5% of 7.5% sodium bicarbonate solution, and 0.001% β-mercaptoethanol. Place the flask in a 37 °C incubator infused with 5.2% CO₂.
2. Harvest the 293T cells using trypsin (0.025%)/EDTA (1 mM) in phosphate-buffered saline (PBS). Add 5 mL of Trypsin/EDTA in PBS and incubate the flasks for 10 min in a 37 °C an incubator to allow the detachment of 293T cells from the T75 cm² flasks.
   1. Collect the detached cells and wash them twice in PBS to remove any traces of trypsin and EDTA. Count the cells with a hemocytometer and adjust the cell number to one million cells per mL.
3. Transfect the 293T cells¹⁸ with 3.95 µg of psPAX2, 1.32 µg of pMD2G, and 5.26 µg of pLEP-GFP-Puro (generated in-house at BRI by cloning EF1alpha promoter from pWPI in place of a CMV promoter in pLenti CMV-GFP-Puro)¹⁹.
   1. Prepare 1 mL of 150 mM NaCl plus 63 µL of 0.6 mg/mL polyethylenimine (PEI; 25,000 kD, linear). Mix well and then add the plasmids and mix. Incubate for 20 min at room temperature and then add it to cells.
   2. 16 h post-transfection, replace the transfection medium with fresh medium plus 4.5 mM sodium butyrate. Harvest the supernatant containing virus 48 h post-transfection and concentrate by overnight centrifugation at 5,000 × g. See the Materials Table.
4. Determine the viral titers using 293T cells by performing serial dilutions and assay by flow cytometry 72 h post-transduction²⁰. Use the expression of green fluorescent protein (GFP) as a measure to quantify the viral titers.

3. Purification and expansion of murine primary NK cells

1. Purify murine primary NK cells²¹. Briefly, pass single-cell spleen suspensions through nylon wool columns to deplete the adherent populations consisting of B cells and macrophages.
2. Culture the non-adherent populations eluted from the nylon wool column that contains murine NK cells with 1,000 U/mL interleukin (IL)-2 in RPMI1640 medium with 10% FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, 5% of 7.5% sodium bicarbonate solution, and 0.001% β-mercaptoethanol (RPMI1640 complete medium).
3. Change the medium from the flasks on day 4 of this culture to remove the non-adherent T and NKT cells; cells that remain adhered to the flasks are largely NK cells. Add 20 mL of fresh RPMI1640 complete medium and 1,000 U/mL IL-2 to replenish the flasks.
4. Check the purity of the murine NK cell cultures on day 7 by flow cytometry with NK cell-specific markers²⁰ using preparations with more than 95% of CD3^-NK1.1⁺ population.

4. Purification and expansion of human primary NK cells

1. Isolate PBMCs by density gradient from the buffy coats of healthy human volunteers. Briefly, carefully layer 35 mL of half-diluted (with HBSS) cells over 15 mL of density gradient in a 50-mL canonical tube. Centrifuge at 400 × g for 30 min at 20 °C in a swinging bucket rotor without brake.
2. Use the commercial antibody-based negative selection kits to purify human primary NK cells according to the manufacturer's protocol.
3. Following the isolation, determine the purity of the NK cell preparations by immunofluorescence analyses. Stain NK cell preparations with 2 µg/mL anti-CD3 and 2 µg/mL anti-CD56 antibodies at 4 °C for 20 min. Wash the cells twice with PBS NK cell populations determined by the absence of CD3 and the presence of CD56 on the cell surface.
4. Incubate the cell preparations with antibodies for 20 min at 4 °C, wash with PBS, and analyze in a flow cytometer. Use NK cell preparations with more than 85% purity for the gene transduction assays²⁰.

5. Transduction of murine and human primary NK cells with lentivirus

1. Suspend mouse or human primary NK cells in a 24-well plate at 0.5 × 10⁵/mL of medium in the presence of GFP lentivirus supernatant at 5, 10, and 20 multiplicity of infection (MOI) and in the presence of Pb (8 µg/mL), PS (8 µg/mL) or dextran (8 µg/mL).
2. Centrifuge the plates at 1,000 × g for 60 min.
3. Without decanting the supernatant, culture the cells overnight (16-18 h) in a 37 °C incubator infused with 5.2% CO_2.
4. Wash with 10 mL of PBS and resuspend in 2 mL of complete RPMI1640 culture medium in the presence of IL-2 (300 U/mL).
5. Test the human and mouse primary NK cell-mediated cytotoxicity against K562 and YAC-1, respectively by performing ⁵¹Chromium (Cr)-release assays at varied effector-to-target-cell ratios²².
   1. Briefly, give one million target cells 50 µCi of radiolabeled sodium chromate ⁵¹Cr. During the 4-h incubation time, they uptake the ⁵¹Cr into cellular proteins. At the end of the incubation, wash the cells to remove any unincorporated label.
   2. Calculate specific tumor cell lysis by the amount of absolute, spontaneous, and experimental release of ⁵¹Cr from the target cells.
      NOTE: The calculation of the percentage of specific lysis from pentaplicate experiments was done using the following equation: % specific lysis = ((⁵¹Cr mean experimental release - ⁵¹Cr mean spontaneous release) / (⁵¹Cr mean maximal release - ⁵¹Cr mean spontaneous release)) х 100, where "⁵¹Cr mean spontaneous release" is the ⁵¹Cr released from target cells in the absence of NK cells and "⁵¹Cr mean maximal release" is the ⁵¹Cr released from target cells upon lysis by 2 N hydrochloric acid (HCl).
6. Harvest the transduced NK cells on day 7 by gently tapping the flasks.
   1. Activate the harvested cells with titrated concentrations of plate-bound anti-NKG2D (A10) mAb by coating 96-well, highly protein-absorbent polystyrene plates with 2.5 µg/mL concentrations of anti-NKG2D mAb overnight.
   2. Wash each well with 100 µL of PBS three times before the addition of primary NK cells.
7. Collect culture supernatants using a multi-channel pipette between 16 and 18 h post-activation to quantify cytokines such as IFN-γ. Generate standard curves using the recombinant cytokines provided with enzyme-linked immunosorbent assay (ELISA) kits.
8. Test the NK cell viability using annexin-V/7-amino-actinomycin D (7-AAD) staining²³ and determine the percent of necrotic cells among the transformed NK cells using a flow cytometer.
   1. To perform this assay, harvest the murine and human NK cells four days after transduction, wash two times with 10 mL of cold PBS at 500 × g for 5 min each, and incubate with an Annexin-V (PE)/7-AAD kit.
9. Use appropriate flow cytometry software to analyze the data. Select the live-cell population and analyze the expression of GFP (FITC channel) as a measure of viral transduction²⁴.

Wyniki

Dextran induces the efficient gene transfer of lentiviral vector in primary human and murine NK cells

Human NK cells were isolated and purified from PBMC (with a purity of more than 85%) and incubated overnight with rIL-2 300 U/mL. These primary NK cells were then transduced with GFP lentivirus at varied multiplicities of infection (MOI; 3, 10, and 20 IU per cell) in 24-well plates in the presence of 8 µg/mL Pb, PS, or dextran...

Dyskusje

This study demonstrates that use of dextran as a cationic polymer agent enhances the lentiviral transduction efficiency of both murine and human primary NK cells. Additionally, other cationic agents, such as Pb or PS, have no discernible effect on the delivery of viral vectors into human primary NK cells. Previously, it has been demonstrated that Pb can augment gene transduction in human T cells¹⁷. These results, however, suggest that neither Pb nor PS have a similar efficiency on human primary NK...

Materiały

Name	Company	Catalog Number	Comments
Dextran	Sigma-Aldrich	90-64-91-9
polybrene (Pb)	Sigma-Aldrich	TR-1003
protamine sulfate (PS)	Sigma-Aldrich	p3369
Trypsin	Corning	25-052-CI
RPMI1640	Corning	10-040-CV
Fetal Bovine Serum	ATALANTA	S11150
Penicillin	Corning	30-001-CI
B-mercaptoethanol	SIGMA	M3148
sodium pyruvate	Corning	MT25000CI
Interferon gamma (IFN-γ )	eBioscience	14-7311-85
Propidium lodding staining solution	BD	51-66211E
Lipofectamine 3000	Thermo Fisher	L3000015
Isoflurane	PHOENIX	NDC 57319-559-05
NK cell negative selection kit	Stem Cell	19855
Yac-1	ATCC	TIB-160
K562	ATCC	CCL-243
Mice	Jakson	664
293T cells	ATCC	CRL-3216
T75 flasks	Cornnig	430641U
antibody-based negative selection kits	Stem Cell	19055
51Chromium (Cr)-release assays	perkin elmer's	NEZ030
ELISA kits	Ebioscience	00-4201-56
Sodium Butyrate	Sigma	5887-5G
Linear polyethylenimine	polysciences	23966-2
Ficoll	GE Life Science	17-1440-03
HBSS	Corning	21-022-CV