Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Podsumowanie

Here we present a closed chest approach to admittance-based bi-ventricular pressure-volume loop recordings in pigs with acute right ventricular dysfunction.

Streszczenie

Pressure-volume (PV) loop recording enables the state-of-the-art investigation of load-independent variables of ventricular performance. Uni-ventricular evaluation is often performed in preclinical research. However, the right and left ventricles exert functional interdependence due to their parallel and serial connections, encouraging simultaneous evaluation of both ventricles. Furthermore, various pharmacological interventions may affect the ventricles and their preloads and afterloads differently.

We describe our closed chest approach to admittance-based bi-ventricular PV loop recordings in a porcine model of acute right ventricular (RV) overload. We utilize minimally invasive techniques with all vascular accesses guided by ultrasound. PV catheters are positioned, under fluoroscopic guidance, to avoid thoracotomy in animals, as the closed chest approach maintains the relevant cardiopulmonary physiology. The admittance technology provides real-time PV loop recordings without the need for post-hoc processing. Furthermore, we explain some essential troubleshooting steps during critical timepoints of the presented procedure.

The presented protocol is a reproducible and physiologically relevant approach to obtain a bi-ventricular cardiac PV loop recording in a large animal model. This can be applied to a large variety of cardiovascular animal research.

Wprowadzenie

Pressure-volume (PV) loops contain a large number of hemodynamic information, including end-systolic and end-diastolic pressures and volumes, ejection fraction, stroke volume, and stroke work¹. Furthermore, transient preload reduction creates a family of loops from which load-independent variables can be derived^2,3. This load-independent evaluation of ventricular function makes PV loop recordings state-of-the-art in hemodynamic evaluation. PV loop recording can be performed in humans but is primarily used and recommended in preclinical research^4,5,6.

Pressure-volume loops can be obtained from both the right ventricle (RV) and the left ventricle (LV). Most research hypotheses are focused on a single ventricle, resulting in only univentricular PV loops being recorded^7,8,9,10. However, the right and left ventricles exert systolic and diastolic interdependence due to their serial and parallel connections within the tight pericardium¹¹. Changes in the output or the size of one ventricle will affect the size, loading conditions, or perfusion of the other ventricle. Thus, bi-ventricular PV loop recordings provide a more comprehensive evaluation of the total cardiac performance. Pharmacological interventions may also affect the two ventricles and their loading conditions differently, further emphasizing the importance of bi-ventricular evaluation.

PV catheters can be advanced into either ventricle by several approaches, including open chest approach with access from the apex of the heart or through the RV outflow tract^{7,10,12,13,14}. However, the opening of the thorax will affect the physiological conditions and may introduce bias.

Based on our experience from previous studies^15,16,17,18, we aim to present our closed chest approach to bi-ventricular PV loop recordings in a large animal model of acute RV failure having minimal influence on cardiopulmonary physiology (Figure 1).

Protokół

This protocol was developed and utilized for studies conducted in compliance with the Danish and Institutional guidelines on animal welfare and ethics. The Danish Animal Research Inspectorate approved the study (license no. 2016-15-0201-00840). A Danish, female slaughter pig (crossbreed of Landrace, Yorkshire, and Duroc) of approximately 60 kg was used.

1. Anesthesia and ventilation

1. Pre-anesthetize the awake pig with Zoletil mix 1 mL/kg (see Table of Materials) as an intramuscular injection to reduce stress, pain, and anxiety of the animal during transportation.
2. Transport the animal from farm facilities to research facilities.
3. Establish intravenous access in an ear vein.
   1. To do so, lightly tourniquet the ear to cause venous blood stasis. Disinfect the skin over a visible, straight vein with ethanol.
   2. Puncture the vein with a 20 G venous catheter and release the tourniquet. Make sure to fix the access with adhesive tape to avoid displacement.
   3. Flush with isotonic saline to ensure the proper positioning of the venous catheter. Observe for slight de-coloring of the vein as the saline passes.
      NOTE: If a subcutaneous bulge appears, the venous catheter is in a subcutaneous position and must be removed. Consider establishing the second intravenous access as a backup.
4. Move the animal to an operating table. Place it in a supine position.
5. Intubate the pig by direct laryngoscopy with a size 7 tube. Fixate the tube to the snout/head of the animal to avoid any accidental extubation. Ensure correct positioning of the tube by looking for equal thoracic movements on ventilation, stethoscopy and/or sufficient expiratory carbon dioxide.
6. Connect the tube to a pre-tested mechanical ventilator and start the ventilation. Use the pressure-controlled, volume-gated ventilation with a tidal volume of 8 mL/kg and low-flow ventilation. The fraction of inspired oxygen (FiO₂) can be 0.21 for normoxia or higher. Adjust the respiratory rate to target the end-tidal carbon dioxide of 5 kPa.
7. Start the total intravenous anesthesia by propofol 3 mg/kg/h and fentanyl 6.25 g/kg/h. Ensure sufficient anesthesia by the lack of corneal reflexes and response to a painful stimulus. Increase infusion, if necessary.
   NOTE: Do not leave the animal unattended at any time until it has regained sufficient consciousness to maintain sternal recumbency (survival protocol) or has been euthanized.
8. Monitor the animal with a 3-lead electrocardiogram and pulse oximetry.
9. Measure the body temperature. If necessary, heat the animal targeting a normal porcine temperature of 38-39 °C.
   NOTE: Hypothermia may increase the risk of arrhythmogenesis triggered by instrumentation¹⁹.
10. Insert the bladder catheter (size 14) by transvaginal access and connect to a urine sampling bag.
11. Depending on the research protocol and the scientific hypothesis to be investigated, consider administering heparin intravenously (5000 IE repeated every 4-6 h, if necessary) and/or amiodaron (300 mg infusion over 20 min).
    NOTE: Heparinization can be performed after intravascular accesses are established. These drugs may ease the instrumentation but might bias results. Alternatively, slow saline infusion on intravenous sheaths can prevent intra-luminal thrombosis.
12. Use vet ointment on the eyes to prevent dryness.

2. Intravascular accesses

NOTE: Intravascular accesses are to be established in the right external jugular vein, the left external jugular vein, left carotid artery, left femoral artery, and right femoral vein. In the pig, the external jugular vein is much larger than the internal jugular vein and, therefore, easier to access. All materials required for this section are shown in Figure 2A.

1. Shave the animal at the sites of puncture for intravascular accesses.
2. Disinfect the skin with chlorhexidine (or povidone iodine) and wipe clean using isopropyl alcohol. Repeat for 2 more cycles.
3. Place a sterile drape at the disinfected area with a centrally located hole in the cover.
4. Use an ultrasound device with a linear probe. Cover the probe with a sterile cover and use sterile gel for vascular examination.
5. Use a 17 G sterile venous catheter to puncture the skin and guide the needle to intravascular positioning by ultrasound (Figure 2B,C).
6. Replace the needle with a guidewire using the Seldinger technique. Remove the venous catheter leaving just the guidewire in the intravascular lumen. Next, make a small skin incision (~5 mm) adherent to the guidewire to ease the insertion of the sheath.
7. Place an 8 French (F) sheath over the guidewire and into the vessel of choice (the Seldinger technique). Choose an 8F sheath in the right external jugular vein (for the right heart catherization) and in the left carotid artery (for LV PV loop catheter). Sufficient lumen is necessary to avoid damaging the catheters.
8. Place a 7F sheath in the left external jugular vein. It will later be exchanged for a larger sheath (see steps 4.4-4.6).
9. Place a 7F sheath in the left femoral artery. The access is for invasive blood pressure measurement and blood gas sampling.
10. Place a 12F (or 14F if available) sheath in the right femoral vein for the inferior vena cava (IVC) balloon insertion. Consider using a dilator in a two-step approach for the larger sheaths.
11. Confirm and control the positioning of all sheaths by drawing blood (venous or arterial, respectively) and easy flushing with isotonic saline. The sheaths are correctly positioned inside a blood vessel if one can draw blood without resistance.
12. Fixate all sheaths with a skin suture (size 3.0) to avoid any accidental removal of a sheath. Skin sutures will be removed after protocol completion along with the removal of sheaths.
13. Connect the femoral arterial access to the pressure transducer and calibrate to atmospheric pressure. Ensure this setup generates the correct form of the arterial pressure curve.
14. Draw an arterial blood sample from an arterial sheath and analyze it on an arterial blood sampler device to evaluate pH, arterial partial pressure of carbon dioxide (PaCO₂), and oxygen (PaO₂, depending on your chosen FiO₂), as well as hemoglobin, electrolytes, blood glucose, and lactate levels.
    1. Correct electrolytes and blood glucose, if necessary, to the standard values by infusion of the needed product. Especially, consider the correction of potassium levels as potassium disturbances may increase the risk of arrhythmogenesis triggered by instrumentation.
15. If the pig was fasting prior to the experiment, consider bolus infusion of isotonic saline (10 mL/kg infused over 30-60 min) or similar crystalloid to counteract hypovolemia.
16. Consider a continuous infusion of 4 mL/kg/h isotonic saline to counteract perspiration throughout the protocol.
    ​NOTE: The experiment can be paused at this step.

3. Right heart catherization

1. Flush a Swan Ganz catheter with saline and ensure the balloon is inflating correctly.
2. Connect the Swan Ganz catheter's ports to the pressure transducers. Reset the pressure to atmospheric pressure holding the two pressure ports (for pulmonary arterial and central venous pressure, respectively) at the mid-axillary level of the pig.
3. Insert the Swan Ganz catheter through the 8F sheath in the right jugular vein (step 2.7).
   CAUTION: Lead aprons or similar protection should be worn whenever using fluoroscopy.
4. Observe on fluoroscopy when the distal part of the Swan Ganz catheter is out of the sheath. Inflate the balloon with the associated syringe.
   NOTE: Inflation of the Swan Ganz balloon inside the sheath will damage the balloon. Anterior-posterior view of fluoroscopy is sufficient for all described procedures.
5. Advance the Swan Ganz catheter slowly following its movements on fluoroscopy. Slower advancements will allow the blood flow to guide the catheter.
6. Observe changes in the pressure signal from the distal port as it enters the RV and shortly after the pulmonary artery (Figure 3). Ensure that the catheter advances without any resistance.
   1. Ensure that the pressure changes from 5-8 mmHg in the central venous circulation to 20-30 mmHg in systole and 0-5 mmHg in diastole in the RV. After passing the pulmonic valves, the diastolic pressure will be 10-15 mmHg (see Figure 3 for changes in shapes of the pressure signal).
      NOTE: Systolic pressures in the RV and in the pulmonary artery above 40 (or a mean pulmonary arterial pressure above 25) might be a sign of pulmonary hypertension due to pneumonic infection in the animal. Please remember that positive pressure mechanical ventilation also may increase pulmonary arterial pressure.
7. Deflate the balloon and ensure that the distal pressure port is still in the main pulmonary artery. Use both fluoroscopy and the pressure signal for this verification.

4. Right ventricular pressure-volume catheter insertion (Figure 4)

1. Read and follow the manufacturer's instructions. Allow the PV catheter to soak in saline for at least 30 min.
2. Open the data acquisition software (see Table of Materials) with an 8-channel setup (pressure, volume, phase, and magnitude from both ventricles). Click Start to ensure the pressure signal is recorded. Look for the excessive noise in the pressure signal. Values will be close to 0 mmHg as the pressure recorder is still outside the animal.
3. Calibrate the pressure to the zero-level by holding the pressure port just below the surface of saline to avoid unwanted pressure effects from the water column above.
4. Insert a long guidewire through the 7F sheath in the left jugular vein (step 2.8). Guided by fluoroscopy, advance the guidewire through the upper central veins, the right atrium (RA), and into the inferior vena cava. Ensure that the advancement is without any resistance. Premature systolic events are common as the guidewire passes the RA.
5. Extract the 7F sheath leaving the guidewire in the venous circulation. Compress the entry point to avoid bleeding. Using the Seldinger technique, exchange the 7F sheath for the 16F sheath. Extend the skin incision for the larger sheath if necessary.
6. Guided by fluoroscopy, advance the 16F sheath over the guidewire until the tip of the sheath (not the dilator) has reached the level of the superior vena cava (Figure 4B).
7. By carefully pulling, extract the dilator and guidewire, but be careful not to remove the sheath. Flush the sheath with isotonic saline to avoid intra-luminal blood clotting.
8. Insert the PV catheter in the 16F sheath.
9. Use fluoroscopy to follow the PV catheter as it passes through the sheath until the pressure-port has left the sheath.
10. Carefully advance the sheath and PV catheter collectively until the sheath is just outside the pericardial border.
11. Advance the PV catheter into the RA (Figure 4C).
12. Use the sheath length to help advance the PV catheter from the RA into the more anteriorly positioned RV; point the external end of the 16F sheath downwards (posterior to the supine animal) and medially, which will point the internal end of the sheath anteriorly.
13. Advance the PV catheter into the RV. This can be verified by the change in pressure-signal from the PV catheter to a classic ventricular shape and by the tactile resistance as the PV catheter meets the right ventricular apex.
14. Once the PV catheter is in the RV, retract the 16F sheath outside the thoracic cavity to avoid any hemodynamic or electrical influence of the device located close to the heart (Figure 4D).
15. Optimize the PV catheter positioning, based on fluoroscopy, as close to the RV apex as possible, but do not let it touch the endocardium.
    NOTE: Use fluoroscopy to observe the excess mechanical contact between PV catheter and endocardium, if any. This is viewed as a bended PV catheter (including its pigtail) and persistent premature systolic events via the electrocardiographic monitoring.
    1. Fixate the PV catheter to the external end of the sheath with adhesion tape to ensure the stability of catheter positioning.
       NOTE: Occasionally, a floating catheter may cause extra-beats. If so, try fixating it without compressing the endocardium too much.
16. Follow the manufacturer's protocol to choose the relevant number of recording segments and to optimize the PV catheter positioning in the RV, based on the recorded phase and magnitude signals.
    ​NOTE: For pigs weighing 60 kg, two or three segments for the RV and most often three segments for the LV were used for this experiment. Fewer segments will be required in smaller animals and vice versa. Positioning of the catheter was based on the magnitude of signals initially; the shape of the pressure-magnitude loop should look like the desired pressure-volume loop. The magnitude amplitude should be as high as possible (5-10 mS). The phase angle should be within 1-3 ^o with the highest possible amplitude (approximately 1.5 ^o).

5. Left ventricular pressure-volume catheter insertion (Figure 5)

1. Read and follow the manufacturer's instructions. Allow the PV catheter to soak in saline for at least 30 min.
2. Calibrate the pressure to zero-level (step 4.3).
3. Insert the PV catheter in the 8F sheath in the left carotid artery.
4. Follow the PV catheter by fluoroscopy as it passes through the sheath towards the aortic valves (Figure 5B). A resistance is felt when the PV catheter is stopped by the aortic valves. On fluoroscopy, bending of the catheter is observed.
   NOTE: Occasionally, the PV catheter turns into the descending aorta. This is recognized by fluoroscopy, and a less prominent aortic notch on the pressure-curve of the PV catheter.
5. Retract the PV catheters approximately 1 cm above the aortic valves.
6. Synchronize the next quick advancement of the PV catheter to a systolic phase of the cardiac cycle. This will happen through the open aortic valves. Success can be verified by the change in the pressure signal from the PV catheter to a classic ventricular shape.
7. If attempts to advance through the valves fail, rotate the PV catheter for better positioning in the center of the ascending aorta. Retry, if needed.
8. Once inside the LV, optimize the left ventricular PV catheter positioning based on fluoroscopy, as close to the LV apex as possible, but do not let it touch the endocardium (Figure 5C). See step 4.15.
   NOTE: Occasionally, a floating catheter may cause premature cardiac contractions. If so, try fixating it without compressing the endocardium too much.
9. Follow manufacturer's protocol to choose the relevant number of recording segments and to optimize the PV catheter positioning in the LV, based on the recorded phase and magnitude signals (see step 4.16).

6. Inferior vena cava balloon insertion

1. Fill the syringe for inflation with saline or contrast agent as preferred and ensure that the balloon can be inflated correctly.
2. Insert the guidewire in the 12F sheath in the right femoral vein.
3. Advance the guidewire to the IVC at the level of the diaphragm.
4. Insert the balloon over the guidewire and advance it to the diaphragm level at the end expiration (Figure 5D).
5. Retract the guidewire and flush the lumen with saline to avoid blood clotting.

7. Pressure-volume catheter calibration

1. Read and follow the manufacturer's instructions.
2. Ensure stable sinus rhythm on the electrocardiographic monitor and stable cardiopulmonary variables for 5-10 min.
3. Use the Swan Ganz catheter to measure the cardiac output (CO) by thermodilution. Use an average of three injections of 10 mL of 5 °C isotonic glucose with less than 10% variation. Observe the animal's heart rate (HR) during the CO measurement. Calculate the stroke volume (SV) as SV = CO/HR (unit mL). Normal CO is 4-6 L/min for a 60 kg pig with a stroke volume of 80-110 mL.
4. Enter the SV into the PV boxes for both the LV and RV.
5. Check that optimal phase and magnitude signals are received from both ventricles. Notably, the two PV boxes must record at different frequencies to avoid electronic cross-talking.
6. In transient apnea, calibrate ("scan") the PV signals.
7. If calibration is satisfactory, ensure the proper shape of both ventricular PV loops, as well as realistic pressures and volumes. If not, re-do the calibration.

8. Baseline evaluation

NOTE: Experiment can be paused at this level for the stabilization of hemodynamics before the research protocol begins.

1. When PV loops are to be recorded, follow the manufacturer's instructions. Press Start in the data acquisition software. Ensure the PV loops are still acceptably shaped.
2. Record PV loops over 30-60 s of continuous ventilation. Perform analysis by finding the average of e.g., three respiratory cycles. Alternatively, perform a transient breath-hold for the end expiration on the ventilator and analyze these loops from apnea. Consider having low/no positive end-expiratory pressure (PEEP) and minimal adjustable pressure limiting (APL) valve.
   NOTE: Ventricular function, especially the RV, is affected by cyclic changes of intrathoracic pressures during ventilation (or spontaneous respiration). Importantly, report in the paper if PV loops were recorded during ventilation or in apnea.
3. For load-independent PV variables, do a breath-hold and wait a few heart beats before slowly inflating the IVC balloon with the chosen liquid (step 6.1). The balloon progressively decreases the cardiac preload.
4. Observe how the RV PV loops become progressively smaller and leftward shifted.
   NOTE: The gradual decrease in RV preload will lower RV end diastolic volume progressively. Lower volumes will cause lower pressures and output (Starling mechanism). For further details, see references^1,2,3.
5. Importantly, keep the balloon inflated by keeping the pressure on the associated syringe long enough for the reduction in LV preload (serially connected with the RV). Observe progressive decrease in LV pressure and volume as well. See the Representative Results section for examples.
6. Quickly, deflate the balloon and turn on ventilation.
7. Re-do 8.3-8.7 if the response was not satisfactory, i.e., without premature cardiac complexes, sinus bradycardia, or similarly affected cardiac function.
8. Allow the pig to stabilize for 2-5 min before the next IVC occlusion.
   NOTE: Hemodynamics are transiently affected by the breath-hold and preload reduction, especially in models of cardiovascular impairment.
9. Consider performing three satisfactory occlusions (see 8.7) to increase the robustness of the statistical analyses.

9. Post Protocol

1. In survival studies, remove and clean all intravascular equipment (PV catheters, IVC balloon, and Swan Ganz catheter).
   1. Cut the skin sutures that kept the sheaths in place. Remove each sheath by manual pulling. Compress on each venous access site for a few minutes to achieve hemostasis.
   2. For arteries, remove the sheath and compress longer (5-10 min) to achieve hemostasis. Alternatively, consider using a vascular closure device.
   3. Close the skin incisions from the sheaths with one adaptive skin suture (3.0, absorbable suture) to avoid bleeding and infection. Apply 5 mL of bupivacaine (5 mg/mL) subcutaneously around each skin incision for pain relief.
2. Once all devices have been removed and hemostasis is achieved, stop the infusion of anesthesia. Carefully observe the animal in this phase.
3. Keep the animal intubated (initially with the cuff inflated) until throat reflexes are present and the animal is sufficiently awake for extubation. Keep measuring the oxygen levels via pulse oximetry before and after extubation to ensure proper ventilation. Apply oxygen if necessary.
4. Do not return the animal to the company of other animals until fully recovered.
5. For survival surgery, maintain proper sterile conditions. Please see steps 2.2-2.5. Observe the skin incisions and sutures daily for signs of infection including measurement of the animal's temperature.
6. Once the experiment ends, perform euthanasia with a lethal dose of pentobarbital (15 mL, 400 mg/mL).

Wyniki

The present instructions describe an approach to achieve admittance-based PV recordings from both the RV and the LV in a large animal.

To compare our simultaneous PV recordings in the RV and LV, we performed a linear regression of the bi-ventricular CO measurements from our largest study¹⁸ with the highest number of simultaneous RV CO and LV CO measurements (n=379 recordings from 12 animals). We found that the slope was 1.03 (95%CI 0.90-1.15) with a Y-intercept of 695 (...

Dyskusje

This paper describes a reproducible minimally invasive closed chest approach for bi-ventricular pressure-volume loop recordings.

Advancement of the PV catheter from the RA into the RV is the most critical step in this protocol. The complex composition of the RV and the stiffness of the catheter complicate insertion into the easily distended and geometrically challenging RV. This difficulty may explain why open chest instrumentation is often preferred. During pilot studies, numerous accesses an...

Materiały

Name	Company	Catalog Number	Comments
12L-RS	GE Healthcare Japan	5141337	Ultrasound probe
12L-RS	GE Healthcare Japan	5141337	Ultrasound probe
Adhesive Aperature Drape (OneMed)	evercare	1515-01	75 x 90 cm (hole: 6 x 8 cm)
Adhesive Aperature Drape (OneMed)	evercare	1515-01	75 x 90 cm (hole: 6 x 8 cm)
Alaris GP Guardrails plus	CareFusion	9002TIG01-G	Infusion pump
Alaris GP Guardrails plus	CareFusion	9002TIG01-G	Infusion pump
Alaris Infusion set	BD Plastipak	60593
Alaris Infusion set	BD Plastipak	60593
Alkoholswap	MEDIQ Danmark	3340012	82% ethanol, 0,5% chlorhexidin, skin disinfection
Alkoholswap	MEDIQ Danmark	3340012	82% ethanol, 0,5% chlorhexidin, skin disinfection
Amplatz Support Wire Guide Extra-Stiff	Cook Medical	THSF-25-260-AES	diameter: 0.025 inches, length: 260 cm
Amplatz Support Wire Guide Extra-Stiff	Cook Medical	THSF-25-260-AES	diameter: 0.025 inches, length: 260 cm
BD Connecta	BD	394601	Luer-Lock
BD Connecta	BD	394601	Luer-Lock
BD Emerald	BD	307736	10 mL syringe
BD Emerald	BD	307736	10 mL syringe
BD Luer-Lock	BD Plastipak	300865	BD = Becton Dickinson, 50 mL syringe
BD Luer-Lock	BD Plastipak	300865	BD = Becton Dickinson, 50 mL syringe
BD Platipak	BD	300613	20 mL syringe
BD Platipak	BD	300613	20 mL syringe
BD Venflon Pro	Becton Dickinson Infusion Therapy	393204	20G
BD Venflon Pro	Becton Dickinson Infusion Therapy	393204	20G
BD Venflon Pro	Becton Dickinson Infusion Therapy	393208	17G
BD Venflon Pro	Becton Dickinson Infusion Therapy	393208	17G
Butomidor Vet	Richter Pharma AG	531943	10 mg/mL
Butomidor Vet	Richter Pharma AG	531943	10 mg/mL
Check-Flo Performer Introducer	Cook Medical	RCFW-16.0P-38-30-RB	16 F sheath, 30 cm long
Check-Flo Performer Introducer	Cook Medical	RCFW-16.0P-38-30-RB	16 F sheath, 30 cm long
Cios Connect S/N 20015	Siemens Healthineers		C-arm
Cios Connect S/N 20015	Siemens Healthineers		C-arm
D-LCC12A-01	GE Healthcare Finland		Pressure measurement monitor
D-LCC12A-01	GE Healthcare Finland		Pressure measurement monitor
Durapore	3M	-	Adhesive tape
Durapore	3M	-	Adhesive tape
E-PRESTIN-00	GE Healthcare Finland	6152932	Respirator tubes
E-PRESTIN-00	GE Healthcare Finland	6152932	Respirator tubes
Exagon vet	Richter Pharma AG	427931	400 mg/mL
Exagon vet	Richter Pharma AG	427931	400 mg/mL
Fast-Cath Hemostasis Introducer 12F	St. Jude Medical	406128	L: 12 cm
Fast-Cath Hemostasis Introducer 12F	St. Jude Medical	406128	L: 12 cm
Favorita II	Aesculap		Type: GT104
Favorita II	Aesculap		Type: GT104
Fentanyl	B. Braun	71036	50 mikrogram/mL
Fentanyl	B. Braun	71036	50 mikrogram/mL
Ketaminol Vet	MSD/Intervet International B.V.	511519	100 mg/mL
Ketaminol Vet	MSD/Intervet International B.V.	511519	100 mg/mL
LabChart	ADInstruments		Data aquisition software
LabChart	ADInstruments		Data aquisition software
Lawton 85-0010 ZK1	Lawton		Laryngoscope
Lawton 85-0010 ZK1	Lawton		Laryngoscope
Lectospiral	VYGON	1159.90	400 cm (Luer-LOCK)
Lectospiral	VYGON	1159.90	400 cm (Luer-LOCK)
Lubrithal eye gel	Dechra, Great Britain
Lubrithal eye gel	Dechra, Great Britain
MBH qufora	MBH-International A/S	13853401	Urine bag
MBH qufora	MBH-International A/S	13853401	Urine bag
Natriumklorid	Fresenius Kabi	7340022100528	9 mg/ml Isotonic saline
Natriumklorid	Fresenius Kabi	7340022100528	9 mg/ml Isotonic saline
PICO50 Aterial Blood Sampler	Radiometer	956-552	2 mL
PICO50 Aterial Blood Sampler	Radiometer	956-552	2 mL
Portex Tracheal Tube	Smiths Medical	100/150/075	"Cuffed Clear Oral/Nasal Murphy Eye"
Portex Tracheal Tube	Smiths Medical	100/150/075	"Cuffed Clear Oral/Nasal Murphy Eye"
PowerLab 16/35	ADInstruments	PL3516	Serial number: 3516-1841
PowerLab 16/35	ADInstruments	PL3516	Serial number: 3516-1841
Pressure Extension set	CODAN	7,14,020	Tube for anesthetics, 150 cm long, inner diameter 0.9 mm
Pressure Extension set	CODAN	7,14,020	Tube for anesthetics, 150 cm long, inner diameter 0.9 mm
Propolipid	Fresenius Kabi	21636	Propofol, 10 mg/mL
Propolipid	Fresenius Kabi	21636	Propofol, 10 mg/mL
PTS-X	NuMED Canada Inc.	PTSX253	Inferior vena cava balloon
PTS-X	NuMED Canada Inc.	PTSX253	Inferior vena cava balloon
Radiofocus Introducer II	Radiofocus/Terumo	RS+B80N10MQ	6+7+8F sheaths
Radiofocus Introducer II	Radiofocus/Terumo	RS+B80N10MQ	6+7+8F sheaths
Rompun Vet	Beyer	86450917	Xylazin, 20 mg/mL
Rompun Vet	Beyer	86450917	Xylazin, 20 mg/mL
Rüsch Brilliant AquaFlate Glycerine	Teleflex	178000	Bladder catheter, size 14
Rüsch Brilliant AquaFlate Glycerine	Teleflex	178000	Bladder catheter, size 14
S/5 Avance	Datex-Ohmeda	-	Mechanical ventilator
S/5 Avance	Datex-Ohmeda	-	Mechanical ventilator
Safersonic Conti Plus & Safergel	SECMA medical innovation	SAF.612.18120.WG.SEC	18 x 120 cm (Safersonic Sterile Transducer Cover with Adhesive Area and Safergel)
Safersonic Conti Plus & Safergel	SECMA medical innovation	SAF.612.18120.WG.SEC	18 x 120 cm (Safersonic Sterile Transducer Cover with Adhesive Area and Safergel)
Scisense Catheter	Transonic Scisense	FDH-5018B-E245B	Serial number: 50-533. Pressure-volume catheter
Scisense Catheter	Transonic Scisense	FDH-5018B-E245B	Serial number: 50-533. Pressure-volume catheter
Scisense Pressure-Volume Measurement System	Transonic Scisense	ADV500	Model: FY097B. Pressure-volume box
Scisense Pressure-Volume Measurement System	Transonic Scisense	ADV500	Model: FY097B. Pressure-volume box
Swan-Ganz CCOmbo	Edwards Lifesciences	744F75	110 cm
Swan-Ganz CCOmbo	Edwards Lifesciences	744F75	110 cm
TruWave Pressure Monitoring Set	Edwards Lifesciences	T434303A	210 cm
TruWave Pressure Monitoring Set	Edwards Lifesciences	T434303A	210 cm
Vivid iq	GE Medical Systems China	Vivid iq
Vivid iq	GE Medical Systems China	Vivid iq
Zoletil 50 Vet (tiletamin 125 mg and zolazepam 125 mg)	Virbac	83046805	Zoletil Mix for pigs: 1 vial of Zoletil 50 Vet (dry matter); add 6.25 mL Xylozin (20 mg/mL), 1.25 mL ketamin (100 mg/mL) and 2.5 mL Butorphanol (10 mg/mL). Dose for pre-anesthesia: 10 mL/10 kg as intramuscular injection
Zoletil 50 Vet (tiletamin 125 mg and zolazepam 125 mg)	Virbac	83046805	Zoletil Mix for pigs: 1 vial of Zoletil 50 Vet (dry matter); add 6.25 mL Xylozin (20 mg/mL), 1.25 mL ketamin (100 mg/mL) and 2.5 mL Butorphanol (10 mg/mL). Dose for pre-anesthesia: 10 mL/10 kg as intramuscular injection