Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Podsumowanie

This article introduces a simple method for expeditious production of giant unilamellar vesicles with encapsulated cytoskeletal proteins. The method proves to be useful for bottom-up reconstitution of cytoskeletal structures in confinement and cytoskeleton-membrane interactions.

Streszczenie

Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes and thus are a great tool to study membrane-related cellular processes in vitro. In recent years, encapsulation within GUVs has proven to be a helpful approach for reconstitution experiments in cell biology and related fields. It better mimics confinement conditions inside living cells, as opposed to conventional biochemical reconstitution. Methods for encapsulation inside GUVs are often not easy to implement, and success rates can differ significantly from lab to lab. One technique that has proven to be successful for encapsulating more complex protein systems is called continuous droplet interface crossing encapsulation (cDICE). Here, a cDICE-based method is presented for rapidly encapsulating cytoskeletal proteins in GUVs with high encapsulation efficiency. In this method, first, lipid-monolayer droplets are generated by emulsifying a protein solution of interest in a lipid/oil mixture. After being added into a rotating 3D-printed chamber, these lipid-monolayered droplets then pass through a second lipid monolayer at a water/oil interface inside the chamber to form GUVs that contain the protein system. This method simplifies the overall procedure of encapsulation within GUVs and speeds up the process, and thus allows us to confine and observe the dynamic evolution of network assembly inside lipid bilayer vesicles. This platform is handy for studying the mechanics of cytoskeleton-membrane interactions in confinement.

Wprowadzenie

Lipid bilayer compartments are used as model synthetic cells for studying enclosed organic reactions and membrane-based processes or as carrier modules in drug delivery applications^1,2. Bottom-up biology with purified components requires minimal experimental systems to explore properties and interactions between biomolecules, such as proteins and lipids^3,4. However, with the advancement of the field, there is an increased need for more complex experimental systems that better imitate the conditions in biological cells. Encapsulation in GUVs is a practical approach that can offer some of these cell-like properties by providing a deformable and selectively permeable lipid bilayer and a confined reaction space. In particular, in vitro reconstitution of cytoskeletal systems, as models of synthetic cells, can benefit from encapsulation in membrane compartments⁵. Many cytoskeletal proteins bind and interact with the cell membrane. As most cytoskeletal assemblies form structures that span the entirety of the cell, their shape is naturally determined by cell-sized confinement⁶.

Different methods are used to generate GUVs, such as the swelling^7,8, small vesicle fusion^9,10, emulsion transfer^11,12, pulsed jetting¹³, and other microfluidic approaches^14,15. Although these methods are still utilized, each has its limitations. Thus, a robust and straightforward approach with a high yield of GUV encapsulation is highly desirable. Although techniques such as spontaneous swelling and electroswelling are widely adopted for the formation of GUVs, these methods are primarily compatible with specific lipid compositions¹⁶, low salt concentration buffers¹⁷, smaller encapsulant molecular size¹⁸, and require a high volume of the encapsulant. Fusing multiple small vesicles into a GUV is inherently energetically unfavorable, thus requiring specificity in charged lipid compositions⁹ and/or external fusion-inducing agents, such as peptides¹⁹ or other chemicals. Emulsion transfer and microfluidic methods, on the other hand, may require droplet stabilization through surfactant and solvent removal after bilayer formation, respectively^18,20. The complexity of experimental setup and device in microfluidic techniques such as pulsed jetting impose an additional challenge²¹. cDICE is an emulsion-based method derived from similar principles governing emulsion transfer^22,23. An aqueous solution (outer solution) and a lipid-oil mixture are stratified by centrifugal forces in a rotating cylindrical chamber (cDICE chamber) forming a lipid saturated interface. Shuttling lipid monolayered aqueous droplets into the rotating cDICE chamber results in zipping of a bilayer as droplets cross the lipid-saturated interface into the outer aqueous solution^22,24. The cDICE approach is a robust technique for GUV encapsulation. With the presented modified method, not only the high vesicle yield typical for cDICE with a significantly shorter encapsulation time (a few seconds) is achieved but GUV generation time that allow for the observation of time-dependent processes (e.g., actin cytoskeletal network formation) is significantly reduced. The protocol takes about 15-20 min from the start to GUV collection and imaging. Here, GUV generation is described using the modified cDICE method for encapsulating actin and actin-binding proteins (ABPs). However, the presented technique is applicable for encapsulating a wide range of biological reactions and membrane interactions, from the assembly of biopolymers to cell-free protein expression to membrane fusion-based cargo transfer.

Protokół

1. Preparation of oil-lipid-mixture

NOTE: The step needs to be performed in a fume hood following all the safety guidelines for handling chloroform.

1. Take 0.5 mL of chloroform in a 15 mL glass vial. Add 88 µL of 25 mg/mL dioleoyl-phosphocholine (DOPC), 9.3 µL of 50 mg/mL cholesterol, and 5 µL of 1 mg/mL dioleoyl-phosphoethanolamine-lissamine rhodamine B (rhodamine PE) (see Table of Materials) into the 15 ml glass vial.
   NOTE: The final mole fractions of DOPC and cholesterol in silicone oil/mineral oil are 69.9% and 30%, respectively. It was established that 20-30 mol% cholesterol is an optimized concentration for membrane fluidity and stability of GUVs generated using the presented technique^25,26. Physiologically, these values are well within the cholesterol concentration range found in mammalian cell plasma membranes⁶. Lipid stocks are acquired as solutions in chloroform and stored at -20 °C. Lipid stock vials should be acclimated to room temperature before they are opened.
2. Pipette 7.2 mL of silicone oil and 1.8 mL of mineral oil in a second 15 mL vial (see Table of Materials). Generally, this needs to be done in a low-humidity glove box, mainly if the mineral oil is reused.
3. Mix the oils by vortexing at the maximum rotational speed (3200 rpm) for 10 s, add the mixture to the vial containing the lipid-in-chloroform mixture and immediately place it on the vortex mixer. Vortex for 10-15 s at the maximum rotational speed (3200 rpm). The resulting lipids-in-oil mix should be slightly cloudy, as the lipids are not fully dissolved in the oil but rather dispersed as small aggregates²⁴.
4. Put the lipid-in-oil dispersion in a bath sonicator (see Table of Materials) with ultrasonic power of 80 W and operating frequency of 40 kHz at room temperature for 30 min. Use the mixture immediately or store at 4 °C for a maximum of 24 h.

2. Vesicle generation

1. Mount the 3D-printed shaft (Supplementary File 1) made from black resin (see Table of Materials) on the benchtop stir plate and set rotational speed to 1200 rpm.
2. Mount the 3D-printed cDICE chamber (Supplementary File 2) made from clear resin (see Table of Materials) on the shaft (Figure 1A,B).
3. Prepare actin and actin-binding proteins (ABP) solutions separately in a total volume of 20 µL.
   1. Prepare 1-10 µM of actin in globular actin buffer (G-buffer), including 10% ATTO 488 actin (see Table of Materials).
      NOTE: 1x G-buffer comprises 5 mM of Tris-HCl, pH 8.0, and 0.2 mM of CaCl₂.
   2. Add filamentous actin polymerization buffer (F-buffer) to begin actin polymerization on ice. Keep the solutions on ice to slow down actin polymerization before the addition of a crosslinker.
      NOTE: The 1x F-buffer contains 50 mM of KCl, 2 mM of MgCl₂, and 3 mM of ATP in 10 mM of Tris, pH 7.5.
   3. Wait for 15 min to allow for initiation of actin polymerization on ice before adding crosslinkers of interest at the desired molar ratio. Keep the solution on ice until encapsulation.
   4. Prepare actin-binding proteins (ABPs) separately in a microtube (Figure 1C).
      NOTE: This step is performed when a combination of ABPs (e.g., myosin, α-actinin, fascin, Arp2/3 complex) encapsulates with actin^25,26,27. In such cases, the desired amount of each ABP is drawn from its stock and added into the microtube designated for ABPs. As the total solution is to be 20 µL, stock aliquots of ABPs must be prepared so that the desired amount of the total ABP mixture does not exceed 5-6 µL. The only ABP used in the representative results here is fascin, the preparation of which is mentioned below.
      1. Aliquot 1.57 µL of fascin from a 1.75 mg/mL of fascin stock (see Table of Materials), and directly add to the actin solution in step 2.7. This corresponds to 2.5 µM of fascin in the solution.
4. Add 7.5% of density gradient medium (see Table of Materials) into the actin solution to create a density gradient between the outer and the inner aqueous phase to facilitate GUV sedimentation.
5. Dispense 700 µL of the outer solution of 200 mM of glucose into the chamber (Figure 1D, left).
   NOTE: The osmolarity of the inner solution determines the concentration of glucose. For these experiments, the osmolarity of the inner solution is ~200 mOsm, so a 200 mM glucose solution is used as the outer solution.
6. Add a sufficient amount of lipid-oil mixture (>3 mL based on the chamber size) into the chamber until 60%-80% of the chamber is filled (Figure 1D, right). An interface will be formed between the lipid-oil mixture and the outer solution.
7. Transfer ABPs (prepared in step 2.3.4) into the actin solution. Using a regular 100-1000 µL pipette, immediately transfer the 700 µL of the lipid-oil mixture into the actin-ABP mixture (Figure 1E, left). Pipette up and down 8 times to generate cell-sized lipid-monolayer droplets with diameter in the range of 7-100 µm (Figure 1E, middle).
   NOTE: Step 2.7 needs to be completed in a few seconds to avoid any actin network assembly before encapsulation. Thus, before performing the step, ensure that pipette tips are already inserted into the pipettes and ready to transfer the mixtures.
8. Using the same 100-1000 µL pipette, immediately dispense the entire emulsion into the rotating chamber. Droplets will acquire a second leaflet of lipids by crossing the lipid monolayer at the oil-outer solution interface, thereby forming GUVs (Figure 1E, right).
9. Remove the chamber from the stir plate and discard most of the lipid-oil mixture by tilting the chamber in the waste container so that a large portion of the lipid-oil mixture is drained from the large opening at the center of the chamber.
   NOTE: This way, the lipid-oil mixture is drawn off the chamber, avoiding mixing of lipid-oil mixture with the outer solution in the next step.
10. Hold the chamber with its lid facing towards the user. Open the chamber lid and slightly tilt the chamber towards the user. The interface between the outer solution containing GUVs and the lipid-oil mixture is visible from the chamber opening (where the lid is located).
11. Using a pipette, collect enough outer solution containing GUVs and dispense 50-300 µL of the outer solution into a 96-well plate to obtain an appropriate density of GUVs.
    NOTE: Following this protocol, a total of about 2 x 10⁵ GUVs are released in the outer solution inside the chamber. The GUV dispersity was not quantified; however, the diameter of about 90% of GUVs is in the range of 12-30 µm. GUVs with any diameter in the range of 7-50 µm can be found in the population. Depending on the encapsulated density gradient medium, GUV size, and solution depth in the well plate, it takes 2-15 min for GUVs to settle down on the surface. The yield of GUVs with reconstituted actin bundles is about 90%.

3. Imaging and 3D image reconstruction

1. Set up the 96 well plate on the stage of an inverted microscope equipped with a spinning disk (or laser scanning) confocal unit, an EMCCD or an sCMOS camera, and an oil immersion 60x objective lens (see Table of Materials).
2. Focus on any region of interest (ROI) and take a z-stack image sequence from the ROI with a z-step interval of 0.5 µm.
   NOTE: Because GUVs can be slightly displaced on the surface over time, it is recommended to capture a multi-wavelength set of images at each z-plane if multiple fluorophores are being imaged, i.e., take a group of 561 nm and 488 nm images at each z-lane at a time to capture ATTO 488 actin and Rhod PE images.
3. Save each z-stack image sequence in .tiff format.
4. Open an image sequence of interest in an image processing software (ImageJ/Fiji). Identify the image with the highest intensity. Hold "ctrl+shift+c" to open the Brightness & Contrast window and click on Reset.
5. From the ImageJ/Fiji menu, go to Analyze > Set Scale and enter the known physical distance and its unit for each image pixel.
6. From the ImageJ/Fiji menu, go to Image > Stacks > 3D project to reconstruct a 3D image from the z-stack. Set "Projection method" as Brightness Point, "Slice Spacing (µm)" as 0.5, and checkmark Interpolate. The default options can be used for the rest of the settings.
   NOTE: z-intervals in some microscopes might not be calibrated, and the actual movement in z-direction might slightly differ from the entered z-interval (i.e., 0.5 µm). In such cases, a 3D calibration specimen such as fluorescent microspheres with a known diameter can be used to obtain the actual z-interval. This value will thus be used as "Slice Spacing (µm)" for 3D projection.

Wyniki

To demonstrate the successful generation of cytoskeletal GUVs using the current protocol, fascin-actin bundle structures in GUVs were reconstituted. Fascin is a short crosslinker of actin filaments which forms stiff parallel-aligned actin bundles and is purified from E. coli as Glutathione-S-Transferase (GST) fusion protein²⁶. 5 µM of actin was first reconstituted, including 0.53 µM of ATTO488 actin in the actin polymerization buffer and 7.5% of the density gradient medium. Upon...

Dyskusje

Different methods of generating GUVs have been explored for the creation of synthetic cells However, the complexity of the procedures, extended time to attain encapsulation, restriction of lipid types and molecular composition of the encapsulant, need for non-physiological chemicals to facilitate encapsulation, low GUV yield, and inconsistencies in encapsulation efficiency have continued to challenge researchers in this field. Considering the wide range of potential studies that can be embarked in bottom-up syntheti...

Materiały

Name	Company	Catalog Number	Comments
18:1 Liss Rhod PE lipid in chloroform	Avanti Polar Lipids	810150C
96 Well Optical Btm Pit PolymerBase	ThermoFisher Scientific	165305
Actin from rabbit skeletal muscle	Cytoskeleton	AKL99-A
ATTO 488-actin from rabbit skeletal muscle	Hypermol	8153-01
Axygen microtubes (200 µL)	Fisher Scientific	14-222-262	for handling ABPs
Black resin	Formlabs	RS-F2-GPBK-04
Cholesterol (powder)	Avanti Polar Lipids	700100P
Choloroform	Sigma Aldrich	67-66-3
Clear resin	Formlabs	RS-F2-GPCL-04
CSU-X1 Confocal Scanner Unit	YOKOGAWA	CSU-X1
Density gradient medium (Optiprep)	Sigma-Aldrich	D1556
DOPC lipid in chloroform	Avanti Polar Lipids	850375C
Fascin	homemade	N/A
F-buffer	homemade	N/A
Fisherbrand microtubes (1.5 mL)	Fisher Scientific	05-408-129
FS02 Sonicator	Fischer Scientific	FS20
G-buffer	homemade	N/A
Glucose	Sigma-Aldrich	158968
iXon X3 camera	Andor	DU-897E-CS0
Mineral oil	Acros Organics	8042-47-5
Olympus IX81 Inverted Microscope	Olympus	IX21
Olympus PlanApo N 60x Oil Microscope Objective	Olumpus	1-U2B933
Silicone oil	Sigma-Aldrich	317667