Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

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6.8K Views

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08:49 min

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February 14th, 2019

DOI :

10.3791/57662-v

February 14th, 2019

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Sara Olivera-Ardid*¹, Nailya Khasbiullina*²^,³, Alexey Nokel³, Andrey Formanovsky², Inna Popova², Tatiana Tyrtysh², Roman Kunetskiy², Nadezhda Shilova², Nicolai Bovin²^,⁴, Daniel Bello-Gil¹, Rafael Mañez¹^,⁵

¹Infectious Pathology and Transplantation Division, Bellvitge Biomedical Research Institute (IDIBELL), ²Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh), Russian Academy of Sciences, ³Semiotik LLC, ⁴Auckland University of Technology, ⁵Intensive Care Department, Bellvitge University Hospital

Podsumowanie

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Keywords Glycan Array

Anti carbohydrate Antibodies

N hydroxy 6 Cinnamic Derivatized Glass Slides

Blocking Buffer

Buffer

Mice Serum

Immunoglobulin Aggregation

Centrifugation

Incubation Chamber

Rozdziały w tym wideo

0:04

Title

0:41

Glycochips Production

1:39

Glycan Array Technique

4:44

Analysis of Glycan Array

6:09

Results: Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in BALB/c Mice

7:26

Conclusion

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