Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

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13.5K Views

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08:25 min

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November 21st, 2018

DOI :

10.3791/58490-v

November 21st, 2018

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Yavuz F. Yazicioglu¹^,², Halil I. Aksoylar¹^,², Rinku Pal¹^,², Nikolaos Patsoukis¹^,², Vassiliki A. Boussiotis¹^,²

¹Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, ²Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School

Podsumowanie

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Keywords Lymphocyte Isolation

Follicular T Helper Cells

Germinal Center B Cells

Intestinal Tissue

Tissue Dissection

Cell Isolation Protocol

Rozdziały w tym wideo

0:04

Title

0:40

Peyer's Patch (PP) Harvest and Single Cell Suspension Preparation

4:08

Surface Marker and Intracellular Protein Staining

6:23

Results: Representative Isolated PP Lymphocyte Population Analyses

8:04

Conclusion

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