Peyer's Patch (PP) Harvest and Single Cell Suspension Preparation
4:08
Surface Marker and Intracellular Protein Staining
6:23
Results: Representative Isolated PP Lymphocyte Population Analyses
8:04
Conclusion
Transcription
This method can answer key questions about the phenotype of mouse Peyer's Patch isolated T and B cell subsets. In particular, follicular T helper and germinal center B cell populations. The main advantage of this technique is that it dispenses wit
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In this study, we present a novel and effective protocol for the isolation of lymphocytes from Peyer's Patches (PPs), which can be subsequently used for in vivo and in vitro functional assays as well as flow cytometric studies of follicular T helper and germinal center B cells.