Swimming Induced Paralysis to Assess Dopamine Signaling in Caenorhabditis elegans

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7.6K Views

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07:36 min

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April 3rd, 2019

DOI :

10.3791/59243-v

April 3rd, 2019

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Sirisha Kudumala¹, Serena Sossi², Lucia Carvelli¹^,³^,⁴

¹Harriet Wilkes Honors College, Florida Atlantic University, John D MacArthur Campus, ²Integrative Biology and Neuroscience program, College of Science, Florida Atlantic University, ³Brain Institute, Florida Atlantic University, ⁴Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University

Podsumowanie

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Swimming Induced Paralysis

SWIP

Dopamine Signaling

Caenorhabditis Elegans

C Elegans

Dopaminergic Synapsis

Extracellular Dopamine

Dopamine Transporter

Synchronized Population

Worm Starter Cultures

Gravids Adults

Worm Lysis

Sodium Hypochlorite Solution

Centrifugation

Embryo Separation

Density Gradient Separation

Rozdziały w tym wideo

0:04

Title

0:48

C. elegans Husbandry

4:12

Swimming-induced Paralysis (SWIP)

6:13

Results: Representative Manual and Automated Amphetamine-induced SWIP Analysis

6:57

Conclusion

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