Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
Please note that all translations are automatically generated. Click here for the English version.
8.9K Views
•
11:33 min
•
March 22nd, 2019
DOI :
March 22nd, 2019
•1Japan Science and Technology Agency, PRESTO, 2Laboratory for Developmental Neurobiology, RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University
Rozdziały w tym wideo
0:04
Title
1:10
Preparation of Poly(ethyleneimine) Coated Coverslips
2:42
Preparation of Rat Cortical Neuron-astrocyte Mixed Culture and Frozen Cells, and the Revival of Frozen Cultures
5:45
Transfection and Adeno-associated Virus Infection of Hippocampal or Cortical Neurons
7:29
Recording Spontaneous Activities of Astrocytes Expressing Lck-RCaMP2 and OER-GCaMP6f
8:37
Results: Dissection of Local Ca2+ Signals in Cultured Cells
10:38
Conclusion
Powiązane Filmy
13:40
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
18.0K Views
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
15.2K Views
10:35
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10.6K Views
08:42
Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm
7.7K Views
10:12
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
12.0K Views
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
10.7K Views
05:56
Imaging Initial Ca2+ Microdomains in Primary T Cells
832 Views
10:05
Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging
7.6K Views
09:34
Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
9.2K Views
07:17
Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
494 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone