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JoVE Journal
Biochemistry
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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
10.6K Views
•
11:36 min
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July 25th, 2019
DOI :
July 25th, 2019
•Rozdziały w tym wideo
0:04
Title
1:01
GSH-agarose Affinity Purification of the GST-RING1B(1-159)-BMI1 (1-109) E3 Ubiquitin Ligase Complex
2:16
Purification of the BAP1/DEUBAD (ASXL2) Deubiquitinase Complex
4:58
Purification of the Nucleosomes from HEK293T Cells
7:38
Nucleosome Ubiquitination Assay Using BMI1/RING1B E3 Ubiquitin Ligase Complex and In Vitro Nucleosomal H2A DUB Assay Using BAP1/DEUBAD
9:05
Results: Purification, Ubiquitination, and Deubiquitination
10:40
Conclusion
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ISSN 2578-2037
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