In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

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12.5K Views

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08:14 min

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July 17th, 2019

DOI :

10.3791/59830-v

July 17th, 2019

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Lina Yu*¹, Wenxiu He*¹, Jie Xie*¹, Rui Guo*¹, Juan Ni², Xia Zhang³, Quishi Xu¹, Caifeng Wang¹, Qiuling Yue¹, Fangfang Li¹, Mengcheng Luo⁴, Bo Sun³, Lan Ye¹, Ke Zheng¹

¹State Key Laboratory of Reproductive Medicine, Nanjing Medical University, ²The Affiliated Hospital of Hangzhou Normal University, ³School of Life Science and Technology, ShanghaiTech University, ⁴Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University

Podsumowanie

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In Vitro Biochemical Assays

Biotin Labels

Protein nucleic Acid Interactions

Reproducibility

Biotin labeled Nucleic Acids

MEIOB

MOV10

Centrifugation

Glutathione Sepharose Beads

Elution Buffer

HEK293T Cells

Anti FLAG Magnetic Beads

Cell Lysis Buffer

Rozdziały w tym wideo

0:04

Title

0:40

Protein Preparation

3:25

Nucleic Acid Preparation and In Vitro Biochemical Assay

5:22

Chemiluminescence Detection

6:18

Results: Interaction of MEIOB and MOV10 Proteins with Nucleic Acids

7:49

Conclusion

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