In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Please note that all translations are automatically generated. Click here for the English version.
12.6K Views
•
08:14 min
•
July 17th, 2019
DOI :
July 17th, 2019
•Lina Yu*1, Wenxiu He*1, Jie Xie*1, Rui Guo*1, Juan Ni2, Xia Zhang3, Quishi Xu1, Caifeng Wang1, Qiuling Yue1, Fangfang Li1, Mengcheng Luo4, Bo Sun3, Lan Ye1, Ke Zheng1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology, ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan University
Rozdziały w tym wideo
0:04
Title
0:40
Protein Preparation
3:25
Nucleic Acid Preparation and In Vitro Biochemical Assay
5:22
Chemiluminescence Detection
6:18
Results: Interaction of MEIOB and MOV10 Proteins with Nucleic Acids
7:49
Conclusion
Powiązane Filmy
13:57
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
29.1K Views
12:03
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
33.5K Views
07:55
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
3.4K Views
08:38
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
22.0K Views
08:07
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
1.2K Views
10:42
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
9.5K Views
07:02
TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
24.0K Views
06:43
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
18.6K Views
09:13
Cell-surface Biotinylation Assay
78.3K Views
01:55
Pomiar aktywności helikazy białka docelowego za pomocą dupleksów RNA znakowanych biotyną
86 Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone