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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

4.0K Views

08:20 min

September 2nd, 2021

DOI :

10.3791/60685-v

September 2nd, 2021


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CRISPR Cas9

Rozdziały w tym wideo

0:04

Introduction

1:00

Lentiviral Preparation

2:47

Determining Virus Titer and Transduction Efficacy with Flow Cytometry

4:27

Lentiviral Transduction of Target Cells and Conditional Induction of Cas9 Mediated Gene Editing

6:06

Results: Analysis of Reversibility and Rapidity of Destabilized DD-Cas9 Protein

7:24

Conclusion

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