Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

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7.8K Views

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08:01 min

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May 12th, 2020

DOI :

10.3791/61154-v

May 12th, 2020

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Kevin Y. Chen¹, Ryan M. Jamiolkowski¹, Alyssa M. Tate¹, Shane A. Fiorenza², Shawn H. Pfeil², Yale E. Goldman¹

¹Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, ²Department of Physics, West Chester University

Podsumowanie

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Zero Mode Waveguides

Nanosphere Lithography

High Concentration Microscopy

Single Molecule Biophysics

Fabrication Protocol

Optical Borosilicate Glass

Acetone Sonication

Potassium Hydroxide Treatment

Micron Polystyrene Beads

Humidity Chamber Setup

Fluorescent Reagents

Coverslip Preparation

Relative Humidity Control

Deposition Techniques

Rozdziały w tym wideo

0:04

Introduction

0:31

Glass Coverslip Cleaning

1:36

Evaporative Deposition of Polystyrene Beads

3:22

Bead Annealing for Reducing Pore Size

3:55

Nanofabrication of Aluminum Zero Mode Waveguides

5:42

Results: ZMW Fabrication Quality Control

7:19

Conclusion

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