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A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

6.2K Views

10:39 min

September 17th, 2020

DOI :

10.3791/61506-v

September 17th, 2020


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Suppressor Screen

Rozdziały w tym wideo

0:04

Introduction

1:24

Identify Potential Genetic Interactions for Screening

2:35

Design the Cloning Strategy to Clone SIR2 into the pRS315 Vector

5:46

Transform the Vector into atg1△ and Wild Type Yeast Strains

7:32

Determine the Chronological Life Span to Test for Shortened CLS Phenotype Suppression

8:13

Results: Chronological Lifespan of atg1△+pRS315-SIR2

10:07

Conclusion

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